Nuclear matrix proteins reflect cell type of origin in cultured human cells. Export

@article{citeulike:1142189, abstract = {The low abundance proteins of the nuclear matrix (NM) were separated from the intermediate filament (IF) proteins and analyzed by two-dimensional gel electrophoresis. Three human breast carcinoma lines had virtually identical patterns of 37 NM proteins. In contrast, cell lines derived from diverse tissues had qualitatively different NM protein patterns. Together, the five cell types examined here had a total of 205 distinguishable NM proteins with 125 of these proteins unique to a single cell type. The remaining NM proteins were shared among cell types to different degrees. Polyclonal antisera, obtained by immunization with total NM proteins as antigens, preferentially stained the nuclear interior and not the exterior IF. These observations suggest that the NM proteins, localized to the interior of the nucleus, vary in a cell-type-specific manner.}, address = {Massachusetts Institute of Technology, Cambridge 02139.}, author = {Fey, E. G. and Penman, S.}, citeulike-article-id = {1142189}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3277168}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3277168}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {human, nuclear\_matrix, nucleus}, month = {January}, number = {1}, pages = {121--125}, posted-at = {2007-03-05 19:41:13}, priority = {2}, title = {Nuclear matrix proteins reflect cell type of origin in cultured human cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3277168}, volume = {85}, year = {1988} }

TY - JOUR ID - citeulike:1142189 L3 - citeulike-article-id:1142189 N2 - The low abundance proteins of the nuclear matrix (NM) were separated from the intermediate filament (IF) proteins and analyzed by two-dimensional gel electrophoresis. Three human breast carcinoma lines had virtually identical patterns of 37 NM proteins. In contrast, cell lines derived from diverse tissues had qualitatively different NM protein patterns. Together, the five cell types examined here had a total of 205 distinguishable NM proteins with 125 of these proteins unique to a single cell type. The remaining NM proteins were shared among cell types to different degrees. Polyclonal antisera, obtained by immunization with total NM proteins as antigens, preferentially stained the nuclear interior and not the exterior IF. These observations suggest that the NM proteins, localized to the interior of the nucleus, vary in a cell-type-specific manner. IS - 1 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Massachusetts Institute of Technology, Cambridge 02139. EP - 125 TI - Nuclear matrix proteins reflect cell type of origin in cultured human cells. VL - 85 SP - 121 KW - human KW - nuclear_matrix KW - nucleus AU - Fey, EG AU - Penman, S PY - 1988/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/3277168 ER -