The enigma of ATCE1, an acrosome-associated transcription factor. Export

@article{citeulike:1178188, abstract = {Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. These results raise the question of why would a transcription factor be specifically anchored to the acrosome inner membrane? An intriguing speculation that ATCE1 might be paternally delivered to the newly formed zygote is discussed.}, address = {The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.}, author = {Gil, S. and Yosef, D. and Golan, N. and Don, J.}, citeulike-article-id = {1178188}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ydbio.2006.06.029}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16925989}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16925989}, doi = {10.1016/j.ydbio.2006.06.029}, issn = {0012-1606}, journal = {Dev Biol}, keywords = {acrosome, atce1, spermatozoa, transcription\_factor, zygote}, month = {October}, number = {1}, pages = {201--211}, posted-at = {2007-03-20 18:50:04}, priority = {2}, title = {The enigma of ATCE1, an acrosome-associated transcription factor.}, url = {http://dx.doi.org/10.1016/j.ydbio.2006.06.029}, volume = {298}, year = {2006} }

TY - JOUR ID - citeulike:1178188 L3 - citeulike-article-id:1178188 N2 - Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. These results raise the question of why would a transcription factor be specifically anchored to the acrosome inner membrane? An intriguing speculation that ATCE1 might be paternally delivered to the newly formed zygote is discussed. IS - 1 JF - Dev Biol SN - 0012-1606 AD - The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. EP - 211 TI - The enigma of ATCE1, an acrosome-associated transcription factor. VL - 298 SP - 201 KW - acrosome KW - atce1 KW - spermatozoa KW - transcription_factor KW - zygote AU - Gil, S AU - Yosef, D AU - Golan, N AU - Don, J PY - 2006/10/01/ UR - http://dx.doi.org/10.1016/j.ydbio.2006.06.029 ER -