On the diversity of sperm histones in the vertebrates: IV. Cytochemical and amino acid analysis in Anura. Export

@article{citeulike:1188293, abstract = {The variability of sperm histones in frogs has been studied by cytochemical and amino acid analyses. Cytochemically, Rana sperm proteins fall into Bloch's ('69, '76) type 4 somatic-like histone category, while Xenopus and Bufo have type 3 intermediate sperm histones. Extractability in 5\% trichloroacetic acid (TCA) at different temperatures splits this type 3 category into two groups: type 3B intermediate sperm histones of Bufo are extractable at 85-90 degrees C, while Xenopus intermediate type 3A sperm histones require temperatures of 95-100 degrees C for extraction. Amino acid analysis confirms that Rana sperm histones are of the nucleosomal type, with a testis-specific, very lysine-rich H1 histone. The sperm protein in Bufo is richer in arginine than the proteins in Xenopus. Both of these genera contain lysine and histidine as well as arginine in their sperm proteins. These results confirm earlier electrophoretic data (Kasinsky et al., '78) and indicate that sperm histones in the order Anura can vary markedly between different genera.}, author = {Kasinsky, H. E. and Huang, S. Y. and Mann, M. and Roca, J. and Subirana, J. A.}, citeulike-article-id = {1188293}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/jez.1402340106}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/3989497}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=3989497}, doi = {10.1002/jez.1402340106}, issn = {0022-104X}, journal = {J Exp Zool}, keywords = {anura, frog, histone, protamine, sperm\_chromatin, spermatozoa, toad}, month = {April}, number = {1}, pages = {33--46}, posted-at = {2007-03-26 17:52:50}, priority = {2}, title = {On the diversity of sperm histones in the vertebrates: IV. Cytochemical and amino acid analysis in Anura.}, url = {http://dx.doi.org/10.1002/jez.1402340106}, volume = {234}, year = {1985} }

TY - JOUR ID - citeulike:1188293 L3 - citeulike-article-id:1188293 N2 - The variability of sperm histones in frogs has been studied by cytochemical and amino acid analyses. Cytochemically, Rana sperm proteins fall into Bloch's ('69, '76) type 4 somatic-like histone category, while Xenopus and Bufo have type 3 intermediate sperm histones. Extractability in 5% trichloroacetic acid (TCA) at different temperatures splits this type 3 category into two groups: type 3B intermediate sperm histones of Bufo are extractable at 85-90 degrees C, while Xenopus intermediate type 3A sperm histones require temperatures of 95-100 degrees C for extraction. Amino acid analysis confirms that Rana sperm histones are of the nucleosomal type, with a testis-specific, very lysine-rich H1 histone. The sperm protein in Bufo is richer in arginine than the proteins in Xenopus. Both of these genera contain lysine and histidine as well as arginine in their sperm proteins. These results confirm earlier electrophoretic data (Kasinsky et al., '78) and indicate that sperm histones in the order Anura can vary markedly between different genera. IS - 1 JF - J Exp Zool SN - 0022-104X EP - 46 TI - On the diversity of sperm histones in the vertebrates: IV. Cytochemical and amino acid analysis in Anura. VL - 234 SP - 33 KW - anura KW - frog KW - histone KW - protamine KW - sperm_chromatin KW - spermatozoa KW - toad AU - Kasinsky, HE AU - Huang, SY AU - Mann, M AU - Roca, J AU - Subirana, JA PY - 1985/04// UR - http://dx.doi.org/10.1002/jez.1402340106 ER -