Sperm nuclear halos can transform into normal chromosomes after injection into oocytes. Export

@article{citeulike:1188533, abstract = {Mouse sperm nuclei extracted with an ionic detergent and 2 M NaCl retain their overall morphology, but upon subsequent reduction of the protamine disulfides they lose all elements of chromatin structure except the organization of DNA into loop that are anchored to the nuclear matrix. These DNA loops appear as a halo surrounding the nuclear matrix, and nuclei extracted in this manner are, therefore, called nuclear halos. Here, we report that sperm nuclear halos injected into oocytes can form pronuclei, then transform into chromosomes with normal morphology. This suggests that sperm nuclear halos retain all the information necessary for normal chromosomal organization, and that micromanipulation of these extracted sperm nuclei can be accomplished without major DNA damage.}, address = {Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Hawaii 96822, USA.}, author = {Mohar, I. and Szczygiel, M. A. and Yanagimachi, R. and Ward, W. S.}, citeulike-article-id = {1188533}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/mrd.10147}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12112607}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12112607}, doi = {10.1002/mrd.10147}, issn = {1040-452X}, journal = {Mol Reprod Dev}, keywords = {chromosome, nuclear\_halo, oocyte, spermatozoa}, month = {July}, number = {3}, pages = {416--420}, posted-at = {2007-03-27 00:10:10}, priority = {2}, title = {Sperm nuclear halos can transform into normal chromosomes after injection into oocytes.}, url = {http://dx.doi.org/10.1002/mrd.10147}, volume = {62}, year = {2002} }

TY - JOUR ID - citeulike:1188533 L3 - citeulike-article-id:1188533 N2 - Mouse sperm nuclei extracted with an ionic detergent and 2 M NaCl retain their overall morphology, but upon subsequent reduction of the protamine disulfides they lose all elements of chromatin structure except the organization of DNA into loop that are anchored to the nuclear matrix. These DNA loops appear as a halo surrounding the nuclear matrix, and nuclei extracted in this manner are, therefore, called nuclear halos. Here, we report that sperm nuclear halos injected into oocytes can form pronuclei, then transform into chromosomes with normal morphology. This suggests that sperm nuclear halos retain all the information necessary for normal chromosomal organization, and that micromanipulation of these extracted sperm nuclei can be accomplished without major DNA damage. IS - 3 JF - Mol Reprod Dev SN - 1040-452X AD - Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Hawaii 96822, USA. EP - 420 TI - Sperm nuclear halos can transform into normal chromosomes after injection into oocytes. VL - 62 SP - 416 KW - chromosome KW - nuclear_halo KW - oocyte KW - spermatozoa AU - Mohar, I AU - Szczygiel, MA AU - Yanagimachi, R AU - Ward, WS PY - 2002/07// UR - http://dx.doi.org/10.1002/mrd.10147 ER -