Expression of proto-oncogenes in mouse eggs and preimplantation embryos. Export

@article{citeulike:1248639, abstract = {The expression of several protooncogenes has been investigated in mouse eggs and preimplantation embryos using reverse transcription coupled to amplification of cDNAs by the polymerase chain reaction (RT-PCR). The genes chosen for analysis included both cytoplasmic (c-raf-1, rasH, rasK, and rasN) and nuclear (c-fos and c-myc) proto-oncogenes encoding proteins involved in the transduction of signals from protein-tyrosine kinase growth factor receptors. Transcripts of the cytoplasmic proto-oncogenes were detected both as maternal and embryonic mRNAs at levels (ca. 1,000 copies per egg or embryo) approximately comparable to their levels of transcription in somatic cells. Transcripts of c-fos and c-myc were also detected in both eggs and embryos, although at more variable levels: Maternal transcripts were present at very low levels (ca. 1-10 copies per egg) in growing oocytes and ovulated eggs; embryonic transcription of c-myc increased, reaching mRNA levels of approximately 100-1,000 copies per embryo in four-cell embryos, morula, and blastocysts; in contrast the transcription of c-fos remained at low, barely detectable levels throughout preimplantation development. Although the significance of the low levels of c-fos mRNA is unclear, these results indicate that preimplantation embryos possess the basic intracellular signaling apparatus required to respond to polypeptide growth factors.}, address = {Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.}, author = {Pal, S. K. and Crowell, R. and Kiessling, A. A. and Cooper, G. M.}, citeulike-article-id = {1248639}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/mrd.1080350103}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/8507485}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=8507485}, doi = {10.1002/mrd.1080350103}, issn = {1040-452X}, journal = {Mol Reprod Dev}, keywords = {c-fos, c-myc, early\_embryo, embryo, mouse, oocyte, preimplantation, proto-oncogene}, month = {May}, number = {1}, pages = {8--15}, posted-at = {2007-04-24 23:28:42}, priority = {2}, title = {Expression of proto-oncogenes in mouse eggs and preimplantation embryos.}, url = {http://dx.doi.org/10.1002/mrd.1080350103}, volume = {35}, year = {1993} }

TY - JOUR ID - citeulike:1248639 L3 - citeulike-article-id:1248639 N2 - The expression of several protooncogenes has been investigated in mouse eggs and preimplantation embryos using reverse transcription coupled to amplification of cDNAs by the polymerase chain reaction (RT-PCR). The genes chosen for analysis included both cytoplasmic (c-raf-1, rasH, rasK, and rasN) and nuclear (c-fos and c-myc) proto-oncogenes encoding proteins involved in the transduction of signals from protein-tyrosine kinase growth factor receptors. Transcripts of the cytoplasmic proto-oncogenes were detected both as maternal and embryonic mRNAs at levels (ca. 1,000 copies per egg or embryo) approximately comparable to their levels of transcription in somatic cells. Transcripts of c-fos and c-myc were also detected in both eggs and embryos, although at more variable levels: Maternal transcripts were present at very low levels (ca. 1-10 copies per egg) in growing oocytes and ovulated eggs; embryonic transcription of c-myc increased, reaching mRNA levels of approximately 100-1,000 copies per embryo in four-cell embryos, morula, and blastocysts; in contrast the transcription of c-fos remained at low, barely detectable levels throughout preimplantation development. Although the significance of the low levels of c-fos mRNA is unclear, these results indicate that preimplantation embryos possess the basic intracellular signaling apparatus required to respond to polypeptide growth factors. IS - 1 JF - Mol Reprod Dev SN - 1040-452X AD - Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115. EP - 15 TI - Expression of proto-oncogenes in mouse eggs and preimplantation embryos. VL - 35 SP - 8 KW - c-fos KW - c-myc KW - early_embryo KW - embryo KW - mouse KW - oocyte KW - preimplantation KW - proto-oncogene AU - Pal, SK AU - Crowell, R AU - Kiessling, AA AU - Cooper, GM PY - 1993/05// UR - http://dx.doi.org/10.1002/mrd.1080350103 ER -