Effect of glutathione depletion on antioxidant enzymes in the epididymis, seminal vesicles, and liver and on spermatozoa motility in the aging brown Norway rat. Export

@article{citeulike:1339421, abstract = {Reactive oxygen species (ROS) play a role in male infertility, where excessive amounts impair spermatozoal motility. Epididymal antioxidant enzymes protect spermatozoa from oxidative damage in the epididymal lumen. Antioxidant secretions from the seminal vesicle protect spermatozoa after ejaculation. As it is known that with age there is increased generation of ROS, the goals of this study were to determine how aging affects the response of antioxidant enzymes in the epididymis, seminal vesicles, and liver to l-buthionine-S,R-sulfoximine (BSO) mediated glutathione (GSH) depletion, and to examine the impact of GSH depletion on motility parameters of spermatozoa from the cauda epididymidis in young (4-mo-old) and old (21-mo-old) rats. Levels of GSH and glutathione disulfide (GSSG), as well as activities of glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase, were measured in the caput, corpus and cauda epididymidis, seminal vesicles, and liver. Spermatozoal motility was assessed by computer-assisted sperm analysis. Significant age-related changes in antioxidant enzyme activities were found in the liver and cauda epididymidis. Glutathione depletion clearly affected tissues in both young and old. The compounding effect of age was most evident in the cauda epididymidis, seminal vesicles, and liver, where antioxidant enzyme activities changed significantly. Additionally, spermatozoa motility was adversely affected after BSO treatment in both age groups, but significantly more so in older animals. In summary, the male reproductive tissues and liver undergo age-related changes in antioxidant enzyme activities and in their response to GSH depletion.}, address = {Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada.}, author = {Zubkova, E. V. and Robaire, B.}, citeulike-article-id = {1339421}, citeulike-linkout-0 = {http://dx.doi.org/10.1095/biolreprod.104.028373}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15151930}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15151930}, doi = {10.1095/biolreprod.104.028373}, issn = {0006-3363}, journal = {Biol Reprod}, keywords = {aging, antioxidant, bso, epididymis, glutathione\_depletion, liver, motility, rat, seminal\_vesicles, spermatozoa}, month = {September}, number = {3}, pages = {1002--1008}, posted-at = {2007-05-28 22:33:33}, priority = {3}, title = {Effect of glutathione depletion on antioxidant enzymes in the epididymis, seminal vesicles, and liver and on spermatozoa motility in the aging brown Norway rat.}, url = {http://dx.doi.org/10.1095/biolreprod.104.028373}, volume = {71}, year = {2004} }

TY - JOUR ID - citeulike:1339421 L3 - citeulike-article-id:1339421 N2 - Reactive oxygen species (ROS) play a role in male infertility, where excessive amounts impair spermatozoal motility. Epididymal antioxidant enzymes protect spermatozoa from oxidative damage in the epididymal lumen. Antioxidant secretions from the seminal vesicle protect spermatozoa after ejaculation. As it is known that with age there is increased generation of ROS, the goals of this study were to determine how aging affects the response of antioxidant enzymes in the epididymis, seminal vesicles, and liver to l-buthionine-S,R-sulfoximine (BSO) mediated glutathione (GSH) depletion, and to examine the impact of GSH depletion on motility parameters of spermatozoa from the cauda epididymidis in young (4-mo-old) and old (21-mo-old) rats. Levels of GSH and glutathione disulfide (GSSG), as well as activities of glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase, were measured in the caput, corpus and cauda epididymidis, seminal vesicles, and liver. Spermatozoal motility was assessed by computer-assisted sperm analysis. Significant age-related changes in antioxidant enzyme activities were found in the liver and cauda epididymidis. Glutathione depletion clearly affected tissues in both young and old. The compounding effect of age was most evident in the cauda epididymidis, seminal vesicles, and liver, where antioxidant enzyme activities changed significantly. Additionally, spermatozoa motility was adversely affected after BSO treatment in both age groups, but significantly more so in older animals. In summary, the male reproductive tissues and liver undergo age-related changes in antioxidant enzyme activities and in their response to GSH depletion. IS - 3 JF - Biol Reprod SN - 0006-3363 AD - Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada. EP - 1008 TI - Effect of glutathione depletion on antioxidant enzymes in the epididymis, seminal vesicles, and liver and on spermatozoa motility in the aging brown Norway rat. VL - 71 SP - 1002 KW - aging KW - antioxidant KW - bso KW - epididymis KW - glutathione_depletion KW - liver KW - motility KW - rat KW - seminal_vesicles KW - spermatozoa AU - Zubkova, EV AU - Robaire, B PY - 2004/09// UR - http://dx.doi.org/10.1095/biolreprod.104.028373 ER -