Concomitant suppression of three target genes can explain the impact of a microRNA on metastasis Export

@article{citeulike:6053642, abstract = {10.1101/gad.1832709 It remains unclear whether a microRNA (miRNA) affects a given phenotype via concomitant down-regulation of its entire repertoire of targets or instead by suppression of only a modest subset of effectors. We demonstrate that inhibition of breast cancer metastasis by miR-31\^{a}€”a miRNA predicted to modulate >200 mRNAs\^{a}€”can be entirely explained by miR-31's pleiotropic regulation of three targets. Thus, concurrent re-expression of integrin-\^{I}±5, radixin, and RhoA abrogates miR-31-imposed metastasis suppression. These effectors influence distinct steps of the metastatic process. Our findings have implications concerning the importance of pleiotropy for the biological actions of miRNAs and provide mechanistic insights into metastasis.}, author = {Valastyan, Scott and Benaich, Nathan and Chang, Amelia and Reinhardt, Ferenc and Weinberg, Robert A.}, citeulike-article-id = {6053642}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gad.1832709}, citeulike-linkout-1 = {http://genesdev.cshlp.org/content/early/2009/10/21/gad.1832709.abstract}, citeulike-linkout-2 = {http://genesdev.cshlp.org/content/early/2009/10/21/gad.1832709.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19875476}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19875476}, day = {29}, doi = {10.1101/gad.1832709}, issn = {1549-5477}, journal = {Genes \& Development}, keywords = {cancer, function, mirna, target}, month = {October}, posted-at = {2009-11-01 15:48:06}, priority = {2}, title = {Concomitant suppression of three target genes can explain the impact of a microRNA on metastasis}, url = {http://dx.doi.org/10.1101/gad.1832709}, year = {2009} }

TY - JOUR ID - citeulike:6053642 L3 - citeulike-article-id:6053642 N2 - 10.1101/gad.1832709 It remains unclear whether a microRNA (miRNA) affects a given phenotype via concomitant down-regulation of its entire repertoire of targets or instead by suppression of only a modest subset of effectors. We demonstrate that inhibition of breast cancer metastasis by miR-31—a miRNA predicted to modulate >200 mRNAs—can be entirely explained by miR-31's pleiotropic regulation of three targets. Thus, concurrent re-expression of integrin-α5, radixin, and RhoA abrogates miR-31-imposed metastasis suppression. These effectors influence distinct steps of the metastatic process. Our findings have implications concerning the importance of pleiotropy for the biological actions of miRNAs and provide mechanistic insights into metastasis. JF - Genes & Development SN - 1549-5477 TI - Concomitant suppression of three target genes can explain the impact of a microRNA on metastasis KW - cancer KW - function KW - mirna KW - target AU - Valastyan, Scott AU - Benaich, Nathan AU - Chang, Amelia AU - Reinhardt, Ferenc AU - Weinberg, Robert PY - 2009/10/29/ UR - http://dx.doi.org/10.1101/gad.1832709 ER -