Shear stress induction of the endothelial nitric oxide synthase gene is calcium-dependent but not calcium-activated Export

@article{xiao1997, abstract = {Arterial levels of shear stress (25 dynes/cm2) can elevate constitutive endothelial nitric oxide synthase (eNOS) gene expression in cultured endothelial cells(Ranjan et al., 1995). By PhosphorImaging of Northern blots, we report that the eNOS/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) ratio in bovine aortic endothelial cells (BAEC) increased by 4.8- and 7.95-fold after 6-hr shear stress exposure of 4 and 25 dynes/cm2, respectively. Incubation of BAEC with dexamethasone (1 \&mgr;M) had no effect on shear stress induction of eNOS mRNA. Buffering of intracellular calcium in BAEC with bis-(o-aminophenoxy)-ethane-N,N,N?, N?-tetraacetic acid, tetra(acetoxymethyl)-ester (BAPTA/AM) reduced shear stress induction of eNOS mRNA by 70\%. Yet, stimulation of BAEC with ionomycin (0.1-1.0 \&mgr;M) for 6-24 hr to elevate intracellular calcium had no effect on eNOS mRNA. These studies indicated that the shear stress induction of eNOS mRNA was a calcium-dependent, but not calcium-activated, process. Shear stress was a very potent and rapid inducer of the eNOS mRNA, which could not be mimicked with phorbol myristrate acetate or endotoxin. Inhibition of tyrosine kinases with genistein (10 \&mgr;M) or tyrphostin B46 (10 \&mgr;M) or inhibition of G-protein signaling with guanosine 5?-O-(2-thiodiphosphate) (GDP-\&bgr;S) (600 \&mgr;M, 6-hr preincubation) did not block the shear stress elevation of eNOS mRNA. J. Cell. Physiol. 171:205-211, 1997. {\copyright} 1997 Wiley-Liss, Inc.}, address = {Bioengineering Laboratory, Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, New York}, author = {Xiao, Zeshuai and Zhang, Zhaohui and Diamond, Scott L.}, citeulike-article-id = {2374179}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/(SICI)1097-4652(199705)171:2%3C205::AID-JCP11%3E3.0.CO;2-C}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/44297/ABSTRACT}, doi = {10.1002/(SICI)1097-4652(199705)171:2%3C205::AID-JCP11%3E3.0.CO;2-C}, journal = {Journal of Cellular Physiology}, keywords = {ca, no, wss}, number = {2}, pages = {205--211}, posted-at = {2008-02-14 14:17:01}, priority = {1}, title = {Shear stress induction of the endothelial nitric oxide synthase gene is calcium-dependent but not calcium-activated}, url = {http://dx.doi.org/10.1002/(SICI)1097-4652(199705)171:2%3C205::AID-JCP11%3E3.0.CO;2-C}, volume = {171}, year = {1997} }

TY - JOUR ID - xiao1997 L3 - citeulike-article-id:2374179 N2 - Arterial levels of shear stress (25 dynes/cm2) can elevate constitutive endothelial nitric oxide synthase (eNOS) gene expression in cultured endothelial cells(Ranjan et al., 1995). By PhosphorImaging of Northern blots, we report that the eNOS/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) ratio in bovine aortic endothelial cells (BAEC) increased by 4.8- and 7.95-fold after 6-hr shear stress exposure of 4 and 25 dynes/cm2, respectively. Incubation of BAEC with dexamethasone (1 &mgr;M) had no effect on shear stress induction of eNOS mRNA. Buffering of intracellular calcium in BAEC with bis-(o-aminophenoxy)-ethane-N,N,N?, N?-tetraacetic acid, tetra(acetoxymethyl)-ester (BAPTA/AM) reduced shear stress induction of eNOS mRNA by 70%. Yet, stimulation of BAEC with ionomycin (0.1-1.0 &mgr;M) for 6-24 hr to elevate intracellular calcium had no effect on eNOS mRNA. These studies indicated that the shear stress induction of eNOS mRNA was a calcium-dependent, but not calcium-activated, process. Shear stress was a very potent and rapid inducer of the eNOS mRNA, which could not be mimicked with phorbol myristrate acetate or endotoxin. Inhibition of tyrosine kinases with genistein (10 &mgr;M) or tyrphostin B46 (10 &mgr;M) or inhibition of G-protein signaling with guanosine 5?-O-(2-thiodiphosphate) (GDP-&bgr;S) (600 &mgr;M, 6-hr preincubation) did not block the shear stress elevation of eNOS mRNA. J. Cell. Physiol. 171:205-211, 1997. © 1997 Wiley-Liss, Inc. IS - 2 JF - Journal of Cellular Physiology AD - Bioengineering Laboratory, Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, New York EP - 211 TI - Shear stress induction of the endothelial nitric oxide synthase gene is calcium-dependent but not calcium-activated VL - 171 SP - 205 KW - ca KW - no KW - wss AU - Xiao, Zeshuai AU - Zhang, Zhaohui AU - Diamond, Scott PY - 1997/// UR - http://dx.doi.org/10.1002/(SICI)1097-4652(199705)171:2%3C205::AID-JCP11%3E3.0.CO;2-C ER -