Stretch-activated whole cell currents in adult rat cardiac myocytes. Export

@article{citeulike:685344, abstract = {Mechanoelectric transduction can initiate cardiac arrhythmias. To examine the origins of this effect at the cellular level, we made whole cell voltage-clamp recordings from acutely isolated rat ventricular myocytes under controlled strain. Longitudinal stretch elicited noninactivating inward cationic currents that increased the action potential duration. These stretch-activated currents could be blocked by 100 microM Gd(3+) but not by octanol. The current-voltage relationship was nearly linear, with a reversal potential of approximately -6 mV in normal Tyrode solution. Current density varied with sarcomere length (SL) according to I (pA/pF) = 8.3 - 5.0 SL (microm). Repeated attempts to record single channel currents from stretch-activated ion channels failed, in accord with the absence of such data from the literature. The inability to record single channel currents may be a result of channels being located on internal membranes such as the T tubules or, possibly, inactivation of the channels by the mechanics of patch formation.}, address = {Department of Physiology and Biophysics, State University of New York, Buffalo, New York 14214, USA.}, author = {Zeng, T. and Bett, G. C. and Sachs, F.}, citeulike-article-id = {685344}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10666087}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10666087}, issn = {0363-6135}, journal = {Am J Physiol Heart Circ Physiol}, keywords = {activated, channel, formulation, stretch}, month = {February}, number = {2}, posted-at = {2006-06-05 23:53:27}, priority = {2}, title = {Stretch-activated whole cell currents in adult rat cardiac myocytes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10666087}, volume = {278}, year = {2000} }

TY - JOUR ID - citeulike:685344 L3 - citeulike-article-id:685344 N2 - Mechanoelectric transduction can initiate cardiac arrhythmias. To examine the origins of this effect at the cellular level, we made whole cell voltage-clamp recordings from acutely isolated rat ventricular myocytes under controlled strain. Longitudinal stretch elicited noninactivating inward cationic currents that increased the action potential duration. These stretch-activated currents could be blocked by 100 microM Gd(3+) but not by octanol. The current-voltage relationship was nearly linear, with a reversal potential of approximately -6 mV in normal Tyrode solution. Current density varied with sarcomere length (SL) according to I (pA/pF) = 8.3 - 5.0 SL (microm). Repeated attempts to record single channel currents from stretch-activated ion channels failed, in accord with the absence of such data from the literature. The inability to record single channel currents may be a result of channels being located on internal membranes such as the T tubules or, possibly, inactivation of the channels by the mechanics of patch formation. IS - 2 JF - Am J Physiol Heart Circ Physiol SN - 0363-6135 AD - Department of Physiology and Biophysics, State University of New York, Buffalo, New York 14214, USA. TI - Stretch-activated whole cell currents in adult rat cardiac myocytes. VL - 278 KW - activated KW - channel KW - formulation KW - stretch AU - Zeng, T AU - Bett, GC AU - Sachs, F PY - 2000/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/10666087 ER -