Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12. Export

@article{paninabordignon97beta2, abstract = {: J Clin Invest. 1997 Sep 15;100(6):1513-9. Links Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12.Panina-Bordignon P, Mazzeo D, Lucia PD, D'Ambrosio D, Lang R, Fabbri L, Self C, Sinigaglia F. Roche Milano Ricerche, I-20132 Milan, Italy. Interleukin 12 (IL-12) plays a central role in the immune system by skewing the immune response towards T helper 1 (Th1) type responses which are characterized by high interferon-gamma and low IL-4 production. In this report we present evidence that beta2-agonists inhibit IL-12 production by both human monocytes in response to lipopolysaccharide (LPS) and dendritic cells stimulated via CD40. Inhibition of IL-12 production is selective, as other cytokines produced by monocytes are unaffected. IL-12 inhibition is dependent on beta2-adrenoceptor stimulation and correlates with increased levels of intracellular cAMP. In conjunction with their ability to suppress IL-12 production, when beta2-agonists are added at priming of neonatal T lymphocytes, they inhibit the development of Th1-type cells, while promoting T helper 2 (Th2) cell differentiation. Further, the in vivo administration of a therapeutic dose of salbutamol results in the selective inhibition of IL-12 production by whole blood lymphocytes stimulated in vitro with LPS. These findings provide new insight into the immunological consequences of the clinical use of beta2-agonists and may suggest new approaches for the treatment of Th1-mediated diseases. PMID: 9294119 [PubMed - indexed for MEDLINE] PMCID: PMC508332}, author = {Bordignon, Panina P. and Mazzeo, D. and Lucia, P. D. and D'Ambrosio, D. and Lang, R. and Fabbri, L. and Self, C. and Sinigaglia, F.}, citeulike-article-id = {3512134}, journal = {Journal of Clinical Investigation}, keywords = {2-ar, effect, ifn-gamma, il-12, on, und}, number = {6}, pages = {1513}, posted-at = {2008-11-14 09:44:44}, priority = {2}, publisher = {American Society for Clinical Investigation}, title = {Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12.}, volume = {100}, year = {1997} }

TY - JOUR ID - paninabordignon97beta2 L3 - citeulike-article-id:3512134 N2 - : J Clin Invest. 1997 Sep 15;100(6):1513-9. Links Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12.Panina-Bordignon P, Mazzeo D, Lucia PD, D'Ambrosio D, Lang R, Fabbri L, Self C, Sinigaglia F. Roche Milano Ricerche, I-20132 Milan, Italy. Interleukin 12 (IL-12) plays a central role in the immune system by skewing the immune response towards T helper 1 (Th1) type responses which are characterized by high interferon-gamma and low IL-4 production. In this report we present evidence that beta2-agonists inhibit IL-12 production by both human monocytes in response to lipopolysaccharide (LPS) and dendritic cells stimulated via CD40. Inhibition of IL-12 production is selective, as other cytokines produced by monocytes are unaffected. IL-12 inhibition is dependent on beta2-adrenoceptor stimulation and correlates with increased levels of intracellular cAMP. In conjunction with their ability to suppress IL-12 production, when beta2-agonists are added at priming of neonatal T lymphocytes, they inhibit the development of Th1-type cells, while promoting T helper 2 (Th2) cell differentiation. Further, the in vivo administration of a therapeutic dose of salbutamol results in the selective inhibition of IL-12 production by whole blood lymphocytes stimulated in vitro with LPS. These findings provide new insight into the immunological consequences of the clinical use of beta2-agonists and may suggest new approaches for the treatment of Th1-mediated diseases. PMID: 9294119 [PubMed - indexed for MEDLINE] PMCID: PMC508332 IS - 6 JF - Journal of Clinical Investigation EP - 1513 TI - Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12. PB - American Society for Clinical Investigation VL - 100 KW - 2-ar KW - effect KW - ifn-gamma KW - il-12 KW - on KW - und AU - Bordignon, Panina AU - Mazzeo, D AU - Lucia, PD AU - D'Ambrosio, D AU - Lang, R AU - Fabbri, L AU - Self, C AU - Sinigaglia, F PY - 1997/// ER -