Mucosal tolerance to E-selectin provides protection against cerebral ischemia--reperfusion injury in rats Export

@article{yun08mucosal, abstract = {1: J Neuroimmunol. 2008 Dec 15;205(1-2):73-9. Epub 2008 Oct 19. Links Mucosal tolerance to E-selectin provides protection against cerebral ischemia-reperfusion injury in rats.Yun W, Qing-Cheng L, Lei Y, Jia-Yin M. Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. OBJECTIVE: To study the effect of mucosal toleration to E-selectin on cerebral ischemia-reperfusion injury in rats and associated mechanisms. METHODS: Rats were exposed to intranasal administration of E-selectin or PBS every other day for 10 days (single-tolerization group) or on two tolerization schedules separated by 11 days (booster-tolerization group). Control group received middle cerebral artery occlusion (MCAO) only. MCAO was performed 48 h after the last dose of E-selectin or PBS. After 2 h ischemia and 22 h reperfusion, the rats were killed. We examined the regional cerebral blood flow, neurological testing, frequencies of CD4+ T and CD8+ T lymphocytes in blood, plasma SOD activity, infarct volumes, and mRNA expressions of IL-10, TGF-beta(1), E-selectin, ICAM-1 and LFA-1 in the ischemic brain tissues. RESULTS: There were 30.25\% (P<0.05) decreases of infarction volumes in the E-selectin booster group accompanied by decreased neurological deficit scores compared with PBS group. Compared with PBS-treated rats, CD8-positive cells were significantly decreased (27.4\%, P<0.05), CD4-positive cells tended to increase (P>0.05), SOD activity was obviously increased (P<0.05), mRNA levels of IL-10 were markedly increased (21.0\%, P<0.05) and TGF-beta(1) showed an upward trend (6.2\%, P>0.05), mRNA levels of E-selectin were prominently decreased (28.7\%, P<0.01) and ICAM-1 and LFA-1 had downward trends (P>0.05) in E-selectin booster animals. CONCLUSIONS: Mucosal tolerance to E-selectin after booster tolerization could relieve cerebral ischemia-reperfusion injury and induce ischemia tolerance in Wistar rats. The mechanisms may involve decreased frequencies of CD8+ T cells in blood, increased plasma SOD activity, heightened mRNA expression of IL-10 and lowered mRNA expression of E-selectin in the ischemic hemisphere. PMID: 18937981 [PubMed - in process]}, author = {Yun, W. and Cheng, Qing L. and Lei, Y. and Yin, Jia M.}, citeulike-article-id = {3815244}, journal = {Journal of Neuroimmunology}, keywords = {e-selectin}, posted-at = {2008-12-21 10:16:29}, priority = {2}, publisher = {Elsevier}, title = {Mucosal tolerance to E-selectin provides protection against cerebral ischemia--reperfusion injury in rats}, year = {2008} }

TY - JOUR ID - yun08mucosal L3 - citeulike-article-id:3815244 N2 - 1: J Neuroimmunol. 2008 Dec 15;205(1-2):73-9. Epub 2008 Oct 19. Links Mucosal tolerance to E-selectin provides protection against cerebral ischemia-reperfusion injury in rats.Yun W, Qing-Cheng L, Lei Y, Jia-Yin M. Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. OBJECTIVE: To study the effect of mucosal toleration to E-selectin on cerebral ischemia-reperfusion injury in rats and associated mechanisms. METHODS: Rats were exposed to intranasal administration of E-selectin or PBS every other day for 10 days (single-tolerization group) or on two tolerization schedules separated by 11 days (booster-tolerization group). Control group received middle cerebral artery occlusion (MCAO) only. MCAO was performed 48 h after the last dose of E-selectin or PBS. After 2 h ischemia and 22 h reperfusion, the rats were killed. We examined the regional cerebral blood flow, neurological testing, frequencies of CD4+ T and CD8+ T lymphocytes in blood, plasma SOD activity, infarct volumes, and mRNA expressions of IL-10, TGF-beta(1), E-selectin, ICAM-1 and LFA-1 in the ischemic brain tissues. RESULTS: There were 30.25% (P<0.05) decreases of infarction volumes in the E-selectin booster group accompanied by decreased neurological deficit scores compared with PBS group. Compared with PBS-treated rats, CD8-positive cells were significantly decreased (27.4%, P<0.05), CD4-positive cells tended to increase (P>0.05), SOD activity was obviously increased (P<0.05), mRNA levels of IL-10 were markedly increased (21.0%, P<0.05) and TGF-beta(1) showed an upward trend (6.2%, P>0.05), mRNA levels of E-selectin were prominently decreased (28.7%, P<0.01) and ICAM-1 and LFA-1 had downward trends (P>0.05) in E-selectin booster animals. CONCLUSIONS: Mucosal tolerance to E-selectin after booster tolerization could relieve cerebral ischemia-reperfusion injury and induce ischemia tolerance in Wistar rats. The mechanisms may involve decreased frequencies of CD8+ T cells in blood, increased plasma SOD activity, heightened mRNA expression of IL-10 and lowered mRNA expression of E-selectin in the ischemic hemisphere. PMID: 18937981 [PubMed - in process] JF - Journal of Neuroimmunology TI - Mucosal tolerance to E-selectin provides protection against cerebral ischemia--reperfusion injury in rats PB - Elsevier KW - e-selectin AU - Yun, W AU - Cheng, Qing AU - Lei, Y AU - Yin, Jia PY - 2008/// ER -