Early Biomarkers of Stroke Export

@misc{reynolds03early, abstract = {1: Clin Chem. 2003 Oct;49(10):1733-9. Links Early biomarkers of stroke.Reynolds MA, Kirchick HJ, Dahlen JR, Anderberg JM, McPherson PH, Nakamura KK, Laskowitz DT, Valkirs GE, Buechler KF. Biosite Incorporated, 11030 Roselle St., San Diego, CA 92121. Duke University Medical Center, Box 2900, Durham, NC 27710, USA. kbuechler@biosite.com BACKGROUND: The diagnosis and management of acute ischemic stroke are limited by the lack of rapid diagnostic assays for use in an emergency setting. Computed tomography (CT) scanning is used to diagnose hemorrhagic stroke but is relatively ineffective (<33\% sensitive) in detecting ischemic stroke. The ability to correlate blood-borne protein biomarkers with stroke phenotypes would aid in the development of such rapid tests. METHODS: ELISAs for >50 protein biomarkers were developed for use on a high-throughput robotic workstation. These assays were used to screen plasma samples from 214 healthy donors and 223 patients diagnosed with stroke, including 82 patients diagnosed with acute ischemic stroke. Marker assay values were first compared by univariate analysis, and then the top markers were subjected to multivariate analysis to derive a marker panel algorithm for the prediction of stroke. RESULTS: The top markers from this analysis were S-100b (a marker of astrocytic activation), B-type neurotrophic growth factor, von Willebrand factor, matrix metalloproteinase-9, and monocyte chemotactic protein-1. In a panel algorithm in which three or more marker values above their respective cutoffs were scored as positive, these five markers provided a sensitivity of 92\% at 93\% specificity for ischemic stroke samples taken within 6 h from symptom onset. CONCLUSION: A marker panel approach to the diagnosis of stroke may provide a useful adjunct to CT scanning in the emergency setting. PMID: 14500614 [PubMed - indexed for MEDLINE]}, author = {Reynolds, M. A. and Kirchick, H. J. and Dahlen, J. R. and Anderberg, J. M. and Mcpherson, P. H. and Nakamura, K. K. and Laskowitz, D. T. and Valkirs, G. E. and Buechler, K. F.}, citeulike-article-id = {3849291}, journal = {Clinical Chemistry}, keywords = {ccl2}, number = {10}, pages = {1733--1739}, posted-at = {2009-01-05 11:30:05}, priority = {2}, publisher = {Am Assoc Clin Chem}, title = {Early Biomarkers of Stroke}, volume = {49}, year = {2003} }

TY - GEN ID - reynolds03early L3 - citeulike-article-id:3849291 N2 - 1: Clin Chem. 2003 Oct;49(10):1733-9. Links Early biomarkers of stroke.Reynolds MA, Kirchick HJ, Dahlen JR, Anderberg JM, McPherson PH, Nakamura KK, Laskowitz DT, Valkirs GE, Buechler KF. Biosite Incorporated, 11030 Roselle St., San Diego, CA 92121. Duke University Medical Center, Box 2900, Durham, NC 27710, USA. kbuechler@biosite.com BACKGROUND: The diagnosis and management of acute ischemic stroke are limited by the lack of rapid diagnostic assays for use in an emergency setting. Computed tomography (CT) scanning is used to diagnose hemorrhagic stroke but is relatively ineffective (<33% sensitive) in detecting ischemic stroke. The ability to correlate blood-borne protein biomarkers with stroke phenotypes would aid in the development of such rapid tests. METHODS: ELISAs for >50 protein biomarkers were developed for use on a high-throughput robotic workstation. These assays were used to screen plasma samples from 214 healthy donors and 223 patients diagnosed with stroke, including 82 patients diagnosed with acute ischemic stroke. Marker assay values were first compared by univariate analysis, and then the top markers were subjected to multivariate analysis to derive a marker panel algorithm for the prediction of stroke. RESULTS: The top markers from this analysis were S-100b (a marker of astrocytic activation), B-type neurotrophic growth factor, von Willebrand factor, matrix metalloproteinase-9, and monocyte chemotactic protein-1. In a panel algorithm in which three or more marker values above their respective cutoffs were scored as positive, these five markers provided a sensitivity of 92% at 93% specificity for ischemic stroke samples taken within 6 h from symptom onset. CONCLUSION: A marker panel approach to the diagnosis of stroke may provide a useful adjunct to CT scanning in the emergency setting. PMID: 14500614 [PubMed - indexed for MEDLINE] IS - 10 JF - Clinical Chemistry EP - 1739 TI - Early Biomarkers of Stroke PB - Am Assoc Clin Chem VL - 49 SP - 1733 KW - ccl2 AU - Reynolds, MA AU - Kirchick, HJ AU - Dahlen, JR AU - Anderberg, JM AU - Mcpherson, PH AU - Nakamura, KK AU - Laskowitz, DT AU - Valkirs, GE AU - Buechler, KF PY - 2003/// ER -