Solubilization of membrane protein complexes for blue native PAGE. Export

@article{citeulike:3038803, abstract = {Blue native PAGE is an electrophoretic technique for high-resolution separation of membrane proteins. The method has been proven especially useful for investigation of native protein complexes enabling a characterization of potential protein-protein interactions in the context of functional proteomics. Blue native PAGE is easy to realise, results are reproducible and a high number of protocols are available. However, care should be taken during solubilization of protein complexes to achieve significant results in BN-PAGE analysis. Solubilization of membranes and proteins is not only influenced by detergent-lipid and detergent-protein interactions but also by lipid-lipid, lipid-protein and protein-protein interactions. Interactions have been investigated experimentally and theoretically. But, in practice, the experimental results do not always mirror the theoretical basis and therefore optimal solubilization conditions for each membrane and membrane protein complex should be investigated individually to tap the full potential of BN-PAGE analysis.}, address = {Department Biology I, Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen, Menzingerstrasse 67, 80638 Munich, Germany.}, author = {Reisinger, V. and Eichacker, L. A.}, citeulike-article-id = {3038803}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jprot.2008.05.004}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1874391908000729}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18573355}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18573355}, day = {21}, doi = {10.1016/j.jprot.2008.05.004}, issn = {1874-3919}, journal = {Journal of proteomics}, keywords = {blue\_native\_gel, bng, membrane\_protein, page}, month = {August}, number = {3}, pages = {277--283}, posted-at = {2009-01-26 11:58:25}, priority = {2}, title = {Solubilization of membrane protein complexes for blue native PAGE.}, url = {http://dx.doi.org/10.1016/j.jprot.2008.05.004}, volume = {71}, year = {2008} }

TY - JOUR ID - citeulike:3038803 L3 - citeulike-article-id:3038803 N2 - Blue native PAGE is an electrophoretic technique for high-resolution separation of membrane proteins. The method has been proven especially useful for investigation of native protein complexes enabling a characterization of potential protein-protein interactions in the context of functional proteomics. Blue native PAGE is easy to realise, results are reproducible and a high number of protocols are available. However, care should be taken during solubilization of protein complexes to achieve significant results in BN-PAGE analysis. Solubilization of membranes and proteins is not only influenced by detergent-lipid and detergent-protein interactions but also by lipid-lipid, lipid-protein and protein-protein interactions. Interactions have been investigated experimentally and theoretically. But, in practice, the experimental results do not always mirror the theoretical basis and therefore optimal solubilization conditions for each membrane and membrane protein complex should be investigated individually to tap the full potential of BN-PAGE analysis. IS - 3 JF - Journal of proteomics SN - 1874-3919 AD - Department Biology I, Ludwig-Maximilians-Universität München, Menzingerstrasse 67, 80638 Munich, Germany. EP - 283 TI - Solubilization of membrane protein complexes for blue native PAGE. VL - 71 SP - 277 KW - blue_native_gel KW - bng KW - membrane_protein KW - page AU - Reisinger, V AU - Eichacker, LA PY - 2008/08/21/ UR - http://dx.doi.org/10.1016/j.jprot.2008.05.004 ER -