Features and applications of blue-native and clear-native electrophoresis. Export

@article{citeulike:3497768, abstract = {1-D native electrophoresis is used for the separation of individual proteins, protein complexes, and supercomplexes. Stable and labile protein-protein interactions can be identified depending on detergent and buffer conditions. 1-D native gels are immediately applicable for in-gel detection of fluorescent-labeled proteins and for in-gel catalytic activity assays. 1-D native gels and blots are used to determine native mass and oligomeric state of membrane proteins. Protein extracts from 1-D native gels are used for generation of antibodies, for proteomic work, and for advanced structural investigations. 2-D separation of subunits of protein complexes by SDS-PAGE is mostly used for immunological and proteomic studies. Following the discussion of these general features, specific applications of native electrophoresis techniques in various research fields are highlighted: immunological and receptor studies, biogenesis and assembly of membrane protein complexes, protein import into organelles, dynamics of proteasomes, proteome and subproteome investigations, the identification and quantification of mitochondrial alterations in apoptosis, carcinogenesis, and neurodegenerative disorders like Parkinson's disease, Alzheimer's disease, and the vast variety of mitochondrial encephalomyopathies.}, address = {Cluster of Excellence ldquoMacromolecular Complexes,rdquo Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Johann Wolfgang Goethe-Universit\"{a}t Frankfurt, Frankfurt am Main, Germany}, author = {Wittig, Ilka and Sch\"{a}gger, Hermann}, citeulike-article-id = {3497768}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/pmic.200800017}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18763698}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18763698}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/121394803/ABSTRACT}, doi = {10.1002/pmic.200800017}, issn = {1615-9861}, journal = {Proteomics}, keywords = {blue\_native\_gel, bng, page}, month = {October}, number = {19}, pages = {3974--3990}, posted-at = {2009-01-26 12:39:02}, priority = {2}, title = {Features and applications of blue-native and clear-native electrophoresis.}, url = {http://dx.doi.org/10.1002/pmic.200800017}, volume = {8}, year = {2008} }

TY - JOUR ID - citeulike:3497768 L3 - citeulike-article-id:3497768 N2 - 1-D native electrophoresis is used for the separation of individual proteins, protein complexes, and supercomplexes. Stable and labile protein-protein interactions can be identified depending on detergent and buffer conditions. 1-D native gels are immediately applicable for in-gel detection of fluorescent-labeled proteins and for in-gel catalytic activity assays. 1-D native gels and blots are used to determine native mass and oligomeric state of membrane proteins. Protein extracts from 1-D native gels are used for generation of antibodies, for proteomic work, and for advanced structural investigations. 2-D separation of subunits of protein complexes by SDS-PAGE is mostly used for immunological and proteomic studies. Following the discussion of these general features, specific applications of native electrophoresis techniques in various research fields are highlighted: immunological and receptor studies, biogenesis and assembly of membrane protein complexes, protein import into organelles, dynamics of proteasomes, proteome and subproteome investigations, the identification and quantification of mitochondrial alterations in apoptosis, carcinogenesis, and neurodegenerative disorders like Parkinson's disease, Alzheimer's disease, and the vast variety of mitochondrial encephalomyopathies. IS - 19 JF - Proteomics SN - 1615-9861 AD - Cluster of Excellence ldquoMacromolecular Complexes,rdquo Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt am Main, Germany EP - 3990 TI - Features and applications of blue-native and clear-native electrophoresis. VL - 8 SP - 3974 KW - blue_native_gel KW - bng KW - page AU - Wittig, Ilka AU - Schägger, Hermann PY - 2008/10// UR - http://dx.doi.org/10.1002/pmic.200800017 ER -