The effects of pressure and cholesterol on rotational motions of perylene in lipid bilayers Export

@article{citeulike:4044072, abstract = {Using steady-state fluorescence polarization measurements, an isothermal pressure-induced phase transition was observed in dimyristoyl- l -α-phosphatidylcholine multilamellar vesicles containing perylene. The temperature-to-pressure equivalence, d T /d P , estimated from the phase transition pressure, , is about 22 K · kbar −1 , which is comparable to values determined from diphenylhexatriene polarization (Chong, P.L.-G. and Weber, G. (1983) Biochemistry 22, 5544–5550). In addition, we have employed a new method, introduced in this paper, to calculate the rate of in-plane rotation ( R ip ) and the rate of out-of-plane rotation ( R op ) of perylene in lipid bilayers. The effects of pressure and cholesterol on the rotational rates of perylene in two lipid bilayer systems have been examined. They are 1-palmitoyl-2-oleoyl- l -α-phosphatidylcholine (POPC) multilamellar vesicles (MLV) and 50 mol\% cholesterol in POPC (MLV). R op is smaller than R op due to the fact that the out-of-plane rotation requires a larger volume change than the in-plane rotation. Cholesterol seems not to affect R op significantly, but pressure causes a decrease in R op by about a factor of three. In contrast, the effects of pressure and cholesterol on R ip are less straightforward. At 1 atm cholesterol increases R ip by a factor of about two. Similarly, in the absence of cholesterol, 1.5 kbar pressure essentially triples R ip . However, if both cholesterol is added and pressure is applied, R ip decreases sharply. The possible interactions between cholesterol and perylene are discussed.}, author = {Chong, P. and Vandermeer, B. and Thompson, T.}, citeulike-article-id = {4044072}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0005-2736(85)90240-8}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/0005273685902408}, day = {14}, doi = {10.1016/0005-2736(85)90240-8}, issn = {00052736}, journal = {Biochimica et Biophysica Acta (BBA) - Biomembranes}, keywords = {bilayer, cholesterol, lipid, liposome}, month = {March}, number = {2}, pages = {253--265}, posted-at = {2009-02-13 15:58:22}, priority = {2}, title = {The effects of pressure and cholesterol on rotational motions of perylene in lipid bilayers}, url = {http://dx.doi.org/10.1016/0005-2736(85)90240-8}, volume = {813}, year = {1985} }

TY - JOUR ID - citeulike:4044072 L3 - citeulike-article-id:4044072 N2 - Using steady-state fluorescence polarization measurements, an isothermal pressure-induced phase transition was observed in dimyristoyl- l -α-phosphatidylcholine multilamellar vesicles containing perylene. The temperature-to-pressure equivalence, d T /d P , estimated from the phase transition pressure, , is about 22 K · kbar −1 , which is comparable to values determined from diphenylhexatriene polarization (Chong, P.L.-G. and Weber, G. (1983) Biochemistry 22, 5544–5550). In addition, we have employed a new method, introduced in this paper, to calculate the rate of in-plane rotation ( R ip ) and the rate of out-of-plane rotation ( R op ) of perylene in lipid bilayers. The effects of pressure and cholesterol on the rotational rates of perylene in two lipid bilayer systems have been examined. They are 1-palmitoyl-2-oleoyl- l -α-phosphatidylcholine (POPC) multilamellar vesicles (MLV) and 50 mol% cholesterol in POPC (MLV). R op is smaller than R op due to the fact that the out-of-plane rotation requires a larger volume change than the in-plane rotation. Cholesterol seems not to affect R op significantly, but pressure causes a decrease in R op by about a factor of three. In contrast, the effects of pressure and cholesterol on R ip are less straightforward. At 1 atm cholesterol increases R ip by a factor of about two. Similarly, in the absence of cholesterol, 1.5 kbar pressure essentially triples R ip . However, if both cholesterol is added and pressure is applied, R ip decreases sharply. The possible interactions between cholesterol and perylene are discussed. IS - 2 JF - Biochimica et Biophysica Acta (BBA) - Biomembranes SN - 00052736 EP - 265 TI - The effects of pressure and cholesterol on rotational motions of perylene in lipid bilayers VL - 813 SP - 253 KW - bilayer KW - cholesterol KW - lipid KW - liposome AU - Chong, P AU - Vandermeer, B AU - Thompson, T PY - 1985/03/14/ UR - http://dx.doi.org/10.1016/0005-2736(85)90240-8 ER -