Stress responses to heterologous membrane protein expression in Escherichia coli Export

@article{citeulike:5135798, abstract = {Abstract\ \ The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.}, author = {Xu, Lucy and James Link, A.}, citeulike-article-id = {5135798}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s10529-009-0075-5}, citeulike-linkout-1 = {http://www.springerlink.com/content/ng6r5422xju74153}, doi = {10.1007/s10529-009-0075-5}, journal = {Biotechnology Letters}, keywords = {expression, membrane\_protein}, posted-at = {2009-07-13 12:55:21}, priority = {2}, title = {Stress responses to heterologous membrane protein expression in Escherichia coli}, url = {http://dx.doi.org/10.1007/s10529-009-0075-5} }

TY - JOUR ID - citeulike:5135798 L3 - citeulike-article-id:5135798 N2 - Abstract  The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms. JF - Biotechnology Letters TI - Stress responses to heterologous membrane protein expression in Escherichia coli KW - expression KW - membrane_protein AU - Xu, Lucy AU - James Link, A UR - http://dx.doi.org/10.1007/s10529-009-0075-5 ER -