Membrane protein expression: no cells required Export

@article{citeulike:5214235, abstract = {Structural and functional studies of membrane proteins have been severely hampered by difficulties in producing sufficient quantities of properly folded protein products. It is well established that cell-based expression of membrane proteins is generally problematic and frequently results in low yield, cell toxicity, protein aggregation and misfolding. Owing to its inherent open nature, cell-free protein expression has become a highly promising tool for the fast and efficient production of these difficult-to-express proteins. Here we review the most recent advances in this field, underscoring the potentials and weaknesses of the newly developed approaches and place specific emphasis on the use of nanolipoprotein particles (NLPs or nanodiscs).}, author = {Katzen, Federico and Peterson, Todd C. and Kudlicki, Wieslaw}, citeulike-article-id = {5214235}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.tibtech.2009.05.005}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0167779909001115}, day = {16}, doi = {10.1016/j.tibtech.2009.05.005}, issn = {01677799}, journal = {Trends in Biotechnology}, keywords = {cell\_free, expression, membrane\_protein}, month = {August}, number = {8}, pages = {455--460}, posted-at = {2009-08-14 12:15:34}, priority = {2}, title = {Membrane protein expression: no cells required}, url = {http://dx.doi.org/10.1016/j.tibtech.2009.05.005}, volume = {27}, year = {2009} }

TY - JOUR ID - citeulike:5214235 L3 - citeulike-article-id:5214235 N2 - Structural and functional studies of membrane proteins have been severely hampered by difficulties in producing sufficient quantities of properly folded protein products. It is well established that cell-based expression of membrane proteins is generally problematic and frequently results in low yield, cell toxicity, protein aggregation and misfolding. Owing to its inherent open nature, cell-free protein expression has become a highly promising tool for the fast and efficient production of these difficult-to-express proteins. Here we review the most recent advances in this field, underscoring the potentials and weaknesses of the newly developed approaches and place specific emphasis on the use of nanolipoprotein particles (NLPs or nanodiscs). IS - 8 JF - Trends in Biotechnology SN - 01677799 EP - 460 TI - Membrane protein expression: no cells required VL - 27 SP - 455 KW - cell_free KW - expression KW - membrane_protein AU - Katzen, Federico AU - Peterson, Todd AU - Kudlicki, Wieslaw PY - 2009/08/16/ UR - http://dx.doi.org/10.1016/j.tibtech.2009.05.005 ER -