Effects of Age and Dietary Fibre Level on Caecal Microbial Communities of Conventional and Specific Pathogen-Free Rabbits Export

@article{citeulike:4375244, abstract = {Caecal community structure in conventional and specific pathogen-free (SPF) rabbits was assessed by performing dot-blot hybridization with 16S rRNA targeted oligonucleotide probes. The variation of the caecal flora was analysed according to age (18-70 days old), nutritional status (acid detergent fibre=19\% in standard and 9\% in fibre-deficient diet) and rabbit health (healthy vs diarrhoeic). Caecal flora was stabilized at around 25-28 days. Bacteria and archaea represented 73\% and 22\%, respectively, of the total microbial communities at weaning (28 days). Cellulolytic bacteria ( Ruminococcus albus , R. flavefaciens , Fibrobacter succinogenes and F. intestinalis ) represented \<7\% of total bacteria, with a predominance of R. flavefaciens and R. albus , respectively, for conventional and SPF rabbits. Bacterial and archaeal rRNAs were twofold higher in conventional than in SPF rabbits, fed standard diet ( p \<0.001). In conventional rabbits, archaeal community was twofold higher with standard diet than with fibre-deficient diet. In both types of rabbits, quantities of Flexibacter-Cytophaga-Bacteroides rRNA were twofold higher with fibre-deficient diet than with standard diet. In diarrhoeic conventional rabbits, the proportion of eukaryal rRNA increased 4-fold, but proportions of bacterial and archaeal rRNA decreased by 1-, 2- and 6-fold, respectively. The quantities of rRNA of F. succinogenes and F. intestinalis in conventional rabbits were, respectively 17 and 5 times higher in sick than in healthy animals, and decreased by 7-fold for R. albus . This study shows some microbial interactions according to nutrition and health of the rabbit.}, author = {Bennegadi, Nadia and Fonty, G\'{e}rard and Millet, Liliane and Gidenne, Thierry and Licois, Dominique}, citeulike-article-id = {4375244}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/08910600310015574}, doi = {10.1080/08910600310015574}, journal = {Microbial Ecology in Health and Disease}, keywords = {16s\_rrna, caecal\_flora, health, nutrition, rabbit}, number = {1}, pages = {23--32}, posted-at = {2009-04-22 13:29:10}, priority = {2}, publisher = {Informa Healthcare}, title = {Effects of Age and Dietary Fibre Level on Caecal Microbial Communities of Conventional and Specific Pathogen-Free Rabbits}, url = {http://dx.doi.org/10.1080/08910600310015574}, volume = {15}, year = {2003} }

TY - JOUR ID - citeulike:4375244 L3 - citeulike-article-id:4375244 N2 - Caecal community structure in conventional and specific pathogen-free (SPF) rabbits was assessed by performing dot-blot hybridization with 16S rRNA targeted oligonucleotide probes. The variation of the caecal flora was analysed according to age (18-70 days old), nutritional status (acid detergent fibre=19% in standard and 9% in fibre-deficient diet) and rabbit health (healthy vs diarrhoeic). Caecal flora was stabilized at around 25-28 days. Bacteria and archaea represented 73% and 22%, respectively, of the total microbial communities at weaning (28 days). Cellulolytic bacteria ( Ruminococcus albus , R. flavefaciens , Fibrobacter succinogenes and F. intestinalis ) represented <7% of total bacteria, with a predominance of R. flavefaciens and R. albus , respectively, for conventional and SPF rabbits. Bacterial and archaeal rRNAs were twofold higher in conventional than in SPF rabbits, fed standard diet ( p <0.001). In conventional rabbits, archaeal community was twofold higher with standard diet than with fibre-deficient diet. In both types of rabbits, quantities of Flexibacter-Cytophaga-Bacteroides rRNA were twofold higher with fibre-deficient diet than with standard diet. In diarrhoeic conventional rabbits, the proportion of eukaryal rRNA increased 4-fold, but proportions of bacterial and archaeal rRNA decreased by 1-, 2- and 6-fold, respectively. The quantities of rRNA of F. succinogenes and F. intestinalis in conventional rabbits were, respectively 17 and 5 times higher in sick than in healthy animals, and decreased by 7-fold for R. albus . This study shows some microbial interactions according to nutrition and health of the rabbit. IS - 1 JF - Microbial Ecology in Health and Disease EP - 32 TI - Effects of Age and Dietary Fibre Level on Caecal Microbial Communities of Conventional and Specific Pathogen-Free Rabbits PB - Informa Healthcare VL - 15 SP - 23 KW - 16s_rrna KW - caecal_flora KW - health KW - nutrition KW - rabbit AU - Bennegadi, Nadia AU - Fonty, Gérard AU - Millet, Liliane AU - Gidenne, Thierry AU - Licois, Dominique PY - 2003/// UR - http://dx.doi.org/10.1080/08910600310015574 ER -