Functional interactions between receptors in bacterial chemotaxis Export

@article{2004aSourjik, abstract = {Bacterial chemotaxis is a model system for signal transduction, noted for its relative simplicity, high sensitivity, wide dynamic range and robustness. Changes in ligand concentrations are sensed by a protein assembly consisting of transmembrane receptors, a coupling protein (CheW) and a histidine kinase (CheA)1, 2, 3, 4. In Escherichia coli, these components are organized at the cell poles in tight clusters that contain several thousand copies of each protein1, 4, 5, 6. Here we studied the effects of variation in the composition of clusters on the activity of the kinase and its sensitivity to attractant stimuli, monitoring responses in vivo using fluorescence resonance energy transfer. Our results indicate that assemblies of bacterial chemoreceptors work in a highly cooperative manner, mimicking the behaviour of allosteric proteins. Conditions that favour steep responses to attractants in mutants with homogeneous receptor populations also enhance the sensitivity of the response in wild-type cells. This is consistent with a number of models7, 8, 9, 10, 11 that assume long-range cooperative interactions between receptors as a general mechanism for signal integration and amplification.}, address = {Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA.}, author = {Sourjik, Victor and Berg, Howard C.}, citeulike-article-id = {2279924}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature02406}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature02406}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15042093}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15042093}, day = {25}, doi = {10.1038/nature02406}, issn = {0028-0836}, journal = {Nature}, keywords = {cluster, fret, mwc, receptor}, month = {March}, number = {6981}, pages = {437--441}, posted-at = {2008-08-07 11:23:59}, priority = {2}, publisher = {Nature Publishing Group}, title = {Functional interactions between receptors in bacterial chemotaxis}, url = {http://dx.doi.org/10.1038/nature02406}, volume = {428}, year = {2004} }

TY - JOUR ID - 2004aSourjik L3 - citeulike-article-id:2279924 N2 - Bacterial chemotaxis is a model system for signal transduction, noted for its relative simplicity, high sensitivity, wide dynamic range and robustness. Changes in ligand concentrations are sensed by a protein assembly consisting of transmembrane receptors, a coupling protein (CheW) and a histidine kinase (CheA)1, 2, 3, 4. In Escherichia coli, these components are organized at the cell poles in tight clusters that contain several thousand copies of each protein1, 4, 5, 6. Here we studied the effects of variation in the composition of clusters on the activity of the kinase and its sensitivity to attractant stimuli, monitoring responses in vivo using fluorescence resonance energy transfer. Our results indicate that assemblies of bacterial chemoreceptors work in a highly cooperative manner, mimicking the behaviour of allosteric proteins. Conditions that favour steep responses to attractants in mutants with homogeneous receptor populations also enhance the sensitivity of the response in wild-type cells. This is consistent with a number of models7, 8, 9, 10, 11 that assume long-range cooperative interactions between receptors as a general mechanism for signal integration and amplification. IS - 6981 JF - Nature SN - 0028-0836 AD - Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA. EP - 441 TI - Functional interactions between receptors in bacterial chemotaxis PB - Nature Publishing Group VL - 428 SP - 437 KW - cluster KW - fret KW - mwc KW - receptor AU - Sourjik, Victor AU - Berg, Howard PY - 2004/03/25/ UR - http://dx.doi.org/10.1038/nature02406 ER -