An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli Export

@article{citeulike:2289539, abstract = {The classical Monod-Wyman-Changeux model for homogeneous allosteric protein complex is generalized in this article to model the responses of heterogeneous receptor complexes to multiple types of ligand stimulus. We show that the recent in vivo experimental data of Escherichia coli chemotaxis responses for mutant strains with different expression levels of the chemo-receptors to different types of stimulus [Sourjik, V. \& Berg, H. C. (2004) Nature 428, 437-441] all can be explained consistently within this generalized Monod-Wyman-Changeux model. Based on the model and the existing data, responses of all of the strains (studied in this article) to the presence of any combinations of ligand (Ser and MeAsp) concentrations are predicted quantitatively for future experimental verification. Through modeling the in vivo response data, our study reveals important information about the properties of different types of individual receptors, as well as the composition of the cluster. The energetic contribution of the nonligand binding, cytoplasmic parts of the cluster, such as CheA and CheW, is also discussed. The generalized allosteric model provides a consistent framework in understanding signal integration and differentiation in bacterial chemotaxis. It should also be useful for studying the functions of other heterogeneous receptor complexes. 10.1073/pnas.0506961102}, author = {Mello, Bernardo A. and Tu, Yuhai}, citeulike-article-id = {2289539}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0506961102}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/102/48/17354}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16293695}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16293695}, day = {29}, doi = {10.1073/pnas.0506961102}, journal = {Proceedings of the National Academy of Sciences}, keywords = {bacterial, chemotaxis, coli, escherichia, mwc, receptor, simulation}, month = {November}, number = {48}, pages = {17354--17359}, posted-at = {2008-01-25 14:50:49}, priority = {0}, title = {An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli}, url = {http://dx.doi.org/10.1073/pnas.0506961102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:2289539 L3 - citeulike-article-id:2289539 N2 - The classical Monod-Wyman-Changeux model for homogeneous allosteric protein complex is generalized in this article to model the responses of heterogeneous receptor complexes to multiple types of ligand stimulus. We show that the recent in vivo experimental data of Escherichia coli chemotaxis responses for mutant strains with different expression levels of the chemo-receptors to different types of stimulus [Sourjik, V. & Berg, H. C. (2004) Nature 428, 437-441] all can be explained consistently within this generalized Monod-Wyman-Changeux model. Based on the model and the existing data, responses of all of the strains (studied in this article) to the presence of any combinations of ligand (Ser and MeAsp) concentrations are predicted quantitatively for future experimental verification. Through modeling the in vivo response data, our study reveals important information about the properties of different types of individual receptors, as well as the composition of the cluster. The energetic contribution of the nonligand binding, cytoplasmic parts of the cluster, such as CheA and CheW, is also discussed. The generalized allosteric model provides a consistent framework in understanding signal integration and differentiation in bacterial chemotaxis. It should also be useful for studying the functions of other heterogeneous receptor complexes. 10.1073/pnas.0506961102 IS - 48 JF - Proceedings of the National Academy of Sciences EP - 17359 TI - An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli VL - 102 SP - 17354 KW - bacterial KW - chemotaxis KW - coli KW - escherichia KW - mwc KW - receptor KW - simulation AU - Mello, Bernardo AU - Tu, Yuhai PY - 2005/11/29/ UR - http://dx.doi.org/10.1073/pnas.0506961102 ER -