Three-dimensional tracking of motile bacteria near a solid planar surface Export

@article{1995Frymier, abstract = {Knowing how motile bacteria move near and along a solid surface is crucial to understanding such diverse phenomena as the migration of infectious bacteria along a catheter, biofilm growth, and the movement of bacteria through the pore spaces of saturated soil, a critical step in the in situ bioremediation of contaminated aquifers. In this study, a tracking microscope is used to record the three-dimensional motion of Escherichia coli near a planar glass surface. Data from the tracking microscope are analyzed to quantify the effects of bacteria-surface interactions on the swimming behavior of bacteria. The speed of cells approaching the surface is found to decrease in agreement with the mathematical model of Ramia et al. [Ramia, M., Tullock, D. L. \& Phan-Tien, N. (1993) Biophys J. 65,755-778], which represents the bacteria as spheres with a single polar flagellum rotating at a constant rate. The tendency of cells to swim adjacent to the surface is shown in computer-generated reproductions of cell traces. The attractive interaction potential between the cells and the solid surface is offered as one of several possible explanations for this tendency.}, author = {Frymier, P. D. and Ford, R. M. and Berg, H. C. and Cummings, P. T.}, citeulike-article-id = {4372454}, citeulike-linkout-0 = {http://www.pnas.org/content/92/13/6195.abstract}, citeulike-linkout-1 = {http://www.pnas.org/content/92/13/6195.full.pdf}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/7597100}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=7597100}, day = {20}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {chemotaxis, tracking}, month = {June}, number = {13}, pages = {6195--6199}, posted-at = {2009-04-21 16:35:35}, priority = {2}, title = {Three-dimensional tracking of motile bacteria near a solid planar surface}, url = {http://www.pnas.org/content/92/13/6195.abstract}, volume = {92}, year = {1995} }

TY - JOUR ID - 1995Frymier L3 - citeulike-article-id:4372454 N2 - Knowing how motile bacteria move near and along a solid surface is crucial to understanding such diverse phenomena as the migration of infectious bacteria along a catheter, biofilm growth, and the movement of bacteria through the pore spaces of saturated soil, a critical step in the in situ bioremediation of contaminated aquifers. In this study, a tracking microscope is used to record the three-dimensional motion of Escherichia coli near a planar glass surface. Data from the tracking microscope are analyzed to quantify the effects of bacteria-surface interactions on the swimming behavior of bacteria. The speed of cells approaching the surface is found to decrease in agreement with the mathematical model of Ramia et al. [Ramia, M., Tullock, D. L. & Phan-Tien, N. (1993) Biophys J. 65,755-778], which represents the bacteria as spheres with a single polar flagellum rotating at a constant rate. The tendency of cells to swim adjacent to the surface is shown in computer-generated reproductions of cell traces. The attractive interaction potential between the cells and the solid surface is offered as one of several possible explanations for this tendency. IS - 13 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 6199 TI - Three-dimensional tracking of motile bacteria near a solid planar surface VL - 92 SP - 6195 KW - chemotaxis KW - tracking AU - Frymier, PD AU - Ford, RM AU - Berg, HC AU - Cummings, PT PY - 1995/06/20/ UR - http://www.pnas.org/content/92/13/6195.abstract ER -