Extreme Clonality in Lymphoblastoid Cell Lines with Implications for Allele Specific Expression Analyses Export

@article{Plagnol_et_al08, abstract = {<p>Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10\% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, <italic>ex vivo</italic> cells for allele specific expression assays.</p>}, author = {Plagnol, Vincent and Uz, Elif and Wallace, Chris and Stevens, Helen and Clayton, David and Ozcelik, Tayfun and Todd, John A.}, citeulike-article-id = {4183644}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pone.0002966}, day = {13}, doi = {10.1371/journal.pone.0002966}, journal = {PLoS ONE}, keywords = {eqtls, geneexpression, lcls}, month = {August}, number = {8}, pages = {e2966+}, posted-at = {2009-03-16 16:13:59}, priority = {2}, publisher = {Public Library of Science}, title = {Extreme Clonality in Lymphoblastoid Cell Lines with Implications for Allele Specific Expression Analyses}, url = {http://dx.doi.org/10.1371/journal.pone.0002966}, volume = {3}, year = {2008} }

TY - JOUR ID - Plagnol_et_al08 L3 - citeulike-article-id:4183644 N2 - <p>Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, <italic>ex vivo</italic> cells for allele specific expression assays.</p> IS - 8 JF - PLoS ONE TI - Extreme Clonality in Lymphoblastoid Cell Lines with Implications for Allele Specific Expression Analyses PB - Public Library of Science VL - 3 SP - e2966 KW - eqtls KW - geneexpression KW - lcls AU - Plagnol, Vincent AU - Uz, Elif AU - Wallace, Chris AU - Stevens, Helen AU - Clayton, David AU - Ozcelik, Tayfun AU - Todd, John PY - 2008/08/13/ UR - http://dx.doi.org/10.1371/journal.pone.0002966 ER -