Autofluorescence of viable cultured mammalian cells. Export

@article{Aubin79, abstract = {The autofluorescence other than intrinsic protein emission of viable cultured mammalian cells has been investigated. The fluorescence was found to originate in discrete cytoplasmic vesicle-like regions and to be absent from the nucleus. Excitation and emission spectra of viable cells revealed at least two distinct fluorescent species. Comparison of cell spectra with spectra of known cellular metabolites suggested that most, if not all, of the fluorescence arises from intracellular nicotinamide adenine dinucleotide (NADH) and riboflavin and flavin coenzymes. Various changes in culture conditions did not affect the observed autofluorescence intensity. A multiparameter flow system (MACCS) was used to compare the fluorescence intensities of numerous cultured mammalian cells.}, author = {Aubin, J. E.}, citeulike-article-id = {4372965}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/220325}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=220325}, issn = {0022-1554}, journal = {The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}, keywords = {facs}, month = {January}, number = {1}, pages = {36--43}, posted-at = {2009-04-21 20:33:10}, priority = {2}, title = {Autofluorescence of viable cultured mammalian cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/220325}, volume = {27}, year = {1979} }

TY - JOUR ID - Aubin79 L3 - citeulike-article-id:4372965 N2 - The autofluorescence other than intrinsic protein emission of viable cultured mammalian cells has been investigated. The fluorescence was found to originate in discrete cytoplasmic vesicle-like regions and to be absent from the nucleus. Excitation and emission spectra of viable cells revealed at least two distinct fluorescent species. Comparison of cell spectra with spectra of known cellular metabolites suggested that most, if not all, of the fluorescence arises from intracellular nicotinamide adenine dinucleotide (NADH) and riboflavin and flavin coenzymes. Various changes in culture conditions did not affect the observed autofluorescence intensity. A multiparameter flow system (MACCS) was used to compare the fluorescence intensities of numerous cultured mammalian cells. IS - 1 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society SN - 0022-1554 EP - 43 TI - Autofluorescence of viable cultured mammalian cells. VL - 27 SP - 36 KW - facs AU - Aubin, JE PY - 1979/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/220325 ER -