Reduction of endogenous angiogenesis inhibitors in Bruch's membrane of the submacular region in eyes with age-related macular degeneration. Export

@article{citeulike:3386068, abstract = {OBJECTIVES: To determine the relative levels of 3 potent inhibitors of angiogenesis (endostatin, pigment epithelium-derived factor, and thrombospondin 1) in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex in the submacular region in aged control eyes and eyes with age-related macular degeneration (AMD). METHODS: Immunohistochemical analysis with antibodies against endostatin, pigment epithelium-derived factor, and thrombospondin 1 was performed on the macular region of aged control donor eyes (n = 8; mean age, 79.8 years) and eyes with AMD (n = 12; mean age, 83.9 years). Three independent masked observers scored the reaction product (scored from 0-7). Mean scores from the control eyes and the eyes with AMD were analyzed using 1-way analysis of variance and unpaired t test. RESULTS: In control eyes, strong immunoreactivity of all 3 inhibitors was observed in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex. Immunoreactivity for endostatin, pigment epithelium-derived factor, and thrombospondin 1 in Bruch's membrane was significantly lower in eyes with AMD compared with aged control eyes (analysis of variance, P = .003, P = .009, and P < .001, respectively). In the choriocapillaris, a significant reduction was observed in endostatin (analysis of variance, P = .02) and thrombospondin 1 (analysis of variance, P = .005) in eyes with AMD. CONCLUSIONS: These findings suggest that endogenous angiogenesis inhibitors in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex may provide a biochemical barrier for choroidal neovascular invasion. CLINICAL RELEVANCE: Decreased levels of angiogenic inhibitors at the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex in eyes with AMD make Bruch's membrane vulnerable to choroidal neovascularization.}, author = {Bhutto, I. A. and Uno, K. and Merges, C. and Zhang, L. and McLeod, D. S. and Lutty, G. A.}, citeulike-article-id = {3386068}, citeulike-linkout-0 = {http://dx.doi.org/10.1001/archopht.126.5.670}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18474778}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18474778}, doi = {10.1001/archopht.126.5.670}, issn = {1538-3601}, journal = {Archives of ophthalmology}, keywords = {cnv, microarray}, month = {May}, number = {5}, pages = {670--678}, posted-at = {2008-10-08 13:30:49}, priority = {5}, title = {Reduction of endogenous angiogenesis inhibitors in Bruch's membrane of the submacular region in eyes with age-related macular degeneration.}, url = {http://dx.doi.org/10.1001/archopht.126.5.670}, volume = {126}, year = {2008} }

TY - JOUR ID - citeulike:3386068 L3 - citeulike-article-id:3386068 N2 - OBJECTIVES: To determine the relative levels of 3 potent inhibitors of angiogenesis (endostatin, pigment epithelium-derived factor, and thrombospondin 1) in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex in the submacular region in aged control eyes and eyes with age-related macular degeneration (AMD). METHODS: Immunohistochemical analysis with antibodies against endostatin, pigment epithelium-derived factor, and thrombospondin 1 was performed on the macular region of aged control donor eyes (n = 8; mean age, 79.8 years) and eyes with AMD (n = 12; mean age, 83.9 years). Three independent masked observers scored the reaction product (scored from 0-7). Mean scores from the control eyes and the eyes with AMD were analyzed using 1-way analysis of variance and unpaired t test. RESULTS: In control eyes, strong immunoreactivity of all 3 inhibitors was observed in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex. Immunoreactivity for endostatin, pigment epithelium-derived factor, and thrombospondin 1 in Bruch's membrane was significantly lower in eyes with AMD compared with aged control eyes (analysis of variance, P = .003, P = .009, and P < .001, respectively). In the choriocapillaris, a significant reduction was observed in endostatin (analysis of variance, P = .02) and thrombospondin 1 (analysis of variance, P = .005) in eyes with AMD. CONCLUSIONS: These findings suggest that endogenous angiogenesis inhibitors in the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex may provide a biochemical barrier for choroidal neovascular invasion. CLINICAL RELEVANCE: Decreased levels of angiogenic inhibitors at the retinal pigment epithelium-Bruch's membrane-choriocapillaris complex in eyes with AMD make Bruch's membrane vulnerable to choroidal neovascularization. IS - 5 JF - Archives of ophthalmology SN - 1538-3601 EP - 678 TI - Reduction of endogenous angiogenesis inhibitors in Bruch's membrane of the submacular region in eyes with age-related macular degeneration. VL - 126 SP - 670 KW - cnv KW - microarray AU - Bhutto, IA AU - Uno, K AU - Merges, C AU - Zhang, L AU - McLeod, DS AU - Lutty, GA PY - 2008/05// UR - http://dx.doi.org/10.1001/archopht.126.5.670 ER -