High Fluidity and Soft Elasticity of the Inner Membrane of Escherichia coli Revealed by the Surface Rheology of Model Langmuir Monolayers Export

@article{lopezmontero08, abstract = {Abstract: We have studied the equilibrium and linear mechanical properties of model membranes of Escherichia coli built up as Langmuir monolayers of a native lipid extract using surface thermodynamics, fluorescence microscopy, and surface rheology measurements. The experimental study has been carried out at different temperatures across the physiological operative range 15-37 C. Lipid phase coexistence has been revealed over a broad pressure range by fluorescence microscopy. The presence of ordered domains has been invoked to explain the emergence of shear elasticity accompanying the hydrostatic compression elasticity typical of fluid monolayers. The surface rheology measurements point out the soft character of E. coli membranes; i.e., upon deformation they react as a near-ideal compliant body with minimal energy dissipation, thus optimizing the effectiveness of external stresses in producing membrane deformations. These mechanical features appear to be independent of temperature, suggesting the existence of a passive thermoregulation mechanism.}, address = {Departamento de Qu\'{\i}mica F\'{\i}sica I, Universidad Complutense de Madrid, 28040 Madrid, Spain, Departamento de Prote\'{\i}nas, Centro de Investigaciones Biol\'{o}gicas, CIB-CSIC, 28040 Madrid, Spain, Departamento de F\'{\i}sica Te\'{o}rica de la Materia Condensada and Instituto de Ciencia de los Materiales Nicols Cabrera, Universidad Aut\'{o}noma de Madrid, 28049 Madrid, Spain}, author = {Lopez-Montero, I. and Arriaga, L. R. and Monroy, F. and Rivas, G. and Tarazona, P. and Velez, M.}, citeulike-article-id = {3212284}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/la703350s}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/la703350s}, comment = {2nd page has great explanation of dilational viscosity and dilational elasticity}, day = {15}, doi = {10.1021/la703350s}, journal = {Langmuir}, keywords = {dilational-viscosity, interface, langmuir}, month = {April}, number = {8}, pages = {4065--4076}, posted-at = {2008-09-10 14:45:41}, priority = {2}, title = {High Fluidity and Soft Elasticity of the Inner Membrane of Escherichia coli Revealed by the Surface Rheology of Model Langmuir Monolayers}, url = {http://dx.doi.org/10.1021/la703350s}, volume = {24}, year = {2008} }

TY - JOUR ID - lopezmontero08 L3 - citeulike-article-id:3212284 N2 - Abstract: We have studied the equilibrium and linear mechanical properties of model membranes of Escherichia coli built up as Langmuir monolayers of a native lipid extract using surface thermodynamics, fluorescence microscopy, and surface rheology measurements. The experimental study has been carried out at different temperatures across the physiological operative range 15-37 C. Lipid phase coexistence has been revealed over a broad pressure range by fluorescence microscopy. The presence of ordered domains has been invoked to explain the emergence of shear elasticity accompanying the hydrostatic compression elasticity typical of fluid monolayers. The surface rheology measurements point out the soft character of E. coli membranes; i.e., upon deformation they react as a near-ideal compliant body with minimal energy dissipation, thus optimizing the effectiveness of external stresses in producing membrane deformations. These mechanical features appear to be independent of temperature, suggesting the existence of a passive thermoregulation mechanism. IS - 8 JF - Langmuir AD - Departamento de Química Física I, Universidad Complutense de Madrid, 28040 Madrid, Spain, Departamento de Proteínas, Centro de Investigaciones Biológicas, CIB-CSIC, 28040 Madrid, Spain, Departamento de Física Teórica de la Materia Condensada and Instituto de Ciencia de los Materiales Nicols Cabrera, Universidad Autónoma de Madrid, 28049 Madrid, Spain EP - 4076 TI - High Fluidity and Soft Elasticity of the Inner Membrane of Escherichia coli Revealed by the Surface Rheology of Model Langmuir Monolayers VL - 24 SP - 4065 KW - dilational-viscosity KW - interface KW - langmuir N1 - 2nd page has great explanation of dilational viscosity and dilational elasticity AU - Lopez-Montero, I AU - Arriaga, LR AU - Monroy, F AU - Rivas, G AU - Tarazona, P AU - Velez, M PY - 2008/04/15/ UR - http://dx.doi.org/10.1021/la703350s ER -