Large-Scale Engineering of the Corynebacterium glutamicum Genome Export

@article{citeulike:1888805, abstract = {The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an improved C. glutamicum genome, we developed a precise genome excision method based on the Cre/loxP recombination system and successfully deleted 11 distinct genomic regions identified by comparative analysis of C. glutamicum genomes. Despite the loss of several predicted open reading frames, the mutant cells exhibited normal growth under standard laboratory conditions. With a total of 250 kb (7.5\% of the genome), the 11 genomic regions were loaded with cryptic prophages, transposons, and genes of unknown function which were dispensable for cell growth, indicating recent horizontal acquisitions to the genome. This provides an interesting background for functional genomic studies and can be used in the improvement of cell traits. 10.1128/AEM.71.6.3369-3372.2005}, author = {Suzuki, Nobuaki and Okayama, Satoshi and Nonaka, Hiroshi and Tsuge, Yota and Inui, Masayuki and Yukawa, Hideaki}, citeulike-article-id = {1888805}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.71.6.3369-3372.2005}, citeulike-linkout-1 = {http://aem.asm.org/cgi/content/abstract/71/6/3369}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15933044}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15933044}, day = {1}, doi = {10.1128/AEM.71.6.3369-3372.2005}, journal = {Appl. Environ. Microbiol.}, keywords = {minimal-genome, minimisation}, month = {June}, number = {6}, pages = {3369--3372}, posted-at = {2007-11-09 09:43:34}, priority = {2}, title = {Large-Scale Engineering of the Corynebacterium glutamicum Genome}, url = {http://dx.doi.org/10.1128/AEM.71.6.3369-3372.2005}, volume = {71}, year = {2005} }

TY - JOUR ID - citeulike:1888805 L3 - citeulike-article-id:1888805 N2 - The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an improved C. glutamicum genome, we developed a precise genome excision method based on the Cre/loxP recombination system and successfully deleted 11 distinct genomic regions identified by comparative analysis of C. glutamicum genomes. Despite the loss of several predicted open reading frames, the mutant cells exhibited normal growth under standard laboratory conditions. With a total of 250 kb (7.5% of the genome), the 11 genomic regions were loaded with cryptic prophages, transposons, and genes of unknown function which were dispensable for cell growth, indicating recent horizontal acquisitions to the genome. This provides an interesting background for functional genomic studies and can be used in the improvement of cell traits. 10.1128/AEM.71.6.3369-3372.2005 IS - 6 JF - Appl. Environ. Microbiol. EP - 3372 TI - Large-Scale Engineering of the Corynebacterium glutamicum Genome VL - 71 SP - 3369 KW - minimal-genome KW - minimisation AU - Suzuki, Nobuaki AU - Okayama, Satoshi AU - Nonaka, Hiroshi AU - Tsuge, Yota AU - Inui, Masayuki AU - Yukawa, Hideaki PY - 2005/06/01/ UR - http://dx.doi.org/10.1128/AEM.71.6.3369-3372.2005 ER -