The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation Export

@article{citeulike:2250541, abstract = {A comparison of catabolic capacity was made between S. cerevisiae cells subjected to 24 h carbon or nitrogen starvation. The cells were shifted to starvation conditions at the onset of respiratory growth on ethanol in aerobic batch cultures, using glucose as the carbon and energy source. The results showed that the catabolic capacity was preserved to a much larger extent during carbon compared to nitrogen starvation. Nitrogen starvation experiments were made in the presence of ethanol (not glucose) to exclude the effect of glucose transport inactivation (Busturia and Lagunas, ). Hence, the difference in catabolic capacity could not be attributed to differences in glucose transport capacity during these conditions. In order to understand the reason for this difference in starvation response, measurement of protein composition, adenine nucleotides, inorganic phosphate, polyphosphate and storage carbohydrates were performed. No clear correlation between any of these variables and catabolic capacity after starvation could be obtained. However, there was a positive correlation between total catabolic activity and intracellular ATP concentration when glucose was added to starved cells. The possible mechanism for this correlation, as well as what determines the ATP level, is discussed. Copyright {\copyright} 2001 John Wiley \& Sons, Ltd.}, address = {Department of Cell and Molecular Biology, G\"{o}teborg University, Box 462, S-405 30 G\"{o}teborg, Sweden; Department of Molecular Biotechnology, Chalmers University of Technology, Box 462, S-405 30 G\"{o}teborg, Sweden; School of Information Science, Computer and Electrical Engineering, Halmstad University, PO Box 823, S-301 18 Halmstad, Sweden}, author = {Nilsson, Annika and P\r{a}hlman, Inga-Lill and Jovall, Per-\r{A}ke and Blomberg, Anders and Larsson, Christer and Gustafsson, Lena}, citeulike-article-id = {2250541}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/yea.786}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/85511491/ABSTRACT}, doi = {10.1002/yea.786}, journal = {Yeast}, keywords = {ferm-capacity, nongrowing}, number = {15}, pages = {1371--1381}, posted-at = {2008-01-18 10:41:44}, priority = {2}, title = {The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation}, url = {http://dx.doi.org/10.1002/yea.786}, volume = {18}, year = {2001} }

TY - JOUR ID - citeulike:2250541 L3 - citeulike-article-id:2250541 N2 - A comparison of catabolic capacity was made between S. cerevisiae cells subjected to 24 h carbon or nitrogen starvation. The cells were shifted to starvation conditions at the onset of respiratory growth on ethanol in aerobic batch cultures, using glucose as the carbon and energy source. The results showed that the catabolic capacity was preserved to a much larger extent during carbon compared to nitrogen starvation. Nitrogen starvation experiments were made in the presence of ethanol (not glucose) to exclude the effect of glucose transport inactivation (Busturia and Lagunas, ). Hence, the difference in catabolic capacity could not be attributed to differences in glucose transport capacity during these conditions. In order to understand the reason for this difference in starvation response, measurement of protein composition, adenine nucleotides, inorganic phosphate, polyphosphate and storage carbohydrates were performed. No clear correlation between any of these variables and catabolic capacity after starvation could be obtained. However, there was a positive correlation between total catabolic activity and intracellular ATP concentration when glucose was added to starved cells. The possible mechanism for this correlation, as well as what determines the ATP level, is discussed. Copyright © 2001 John Wiley & Sons, Ltd. IS - 15 JF - Yeast AD - Department of Cell and Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden; Department of Molecular Biotechnology, Chalmers University of Technology, Box 462, S-405 30 Göteborg, Sweden; School of Information Science, Computer and Electrical Engineering, Halmstad University, PO Box 823, S-301 18 Halmstad, Sweden EP - 1381 TI - The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation VL - 18 SP - 1371 KW - ferm-capacity KW - nongrowing AU - Nilsson, Annika AU - Påhlman, Inga-Lill AU - Jovall, Per-Åke AU - Blomberg, Anders AU - Larsson, Christer AU - Gustafsson, Lena PY - 2001/// UR - http://dx.doi.org/10.1002/yea.786 ER -