Global metabolite analysis of yeast: evaluation of sample preparation methods Export

@article{citeulike:2393771, abstract = {Sample preparation is considered one of the limiting steps in microbial metabolome analysis. Eukaryotes and prokaryotes behave very differently during the several steps of classical sample preparation methods for analysis of metabolites. Even within the eukaryote kingdom there is a vast diversity of cell structures that make it imprudent to blindly adopt protocols that were designed for a specific group of microorganisms. We have therefore reviewed and evaluated the whole sample preparation procedures for analysis of yeast metabolites. Our focus has been on the current needs in metabolome analysis, which is the analysis of a large number of metabolites with very diverse chemical and physical properties. This work reports the leakage of intracellular metabolites observed during quenching yeast cells with cold methanol solution, the efficacy of six different methods for the extraction of intracellular metabolites, and the losses noticed during sample concentration by lyophilization and solvent evaporation. A more reliable procedure is suggested for quenching yeast cells with cold methanol solution, followed by extraction of intracellular metabolites by pure methanol. The method can be combined with reduced pressure solvent evaporation and therefore represents an attractive sample preparation procedure for high-throughput metabolome analysis of yeasts. Copyright {\copyright} 2005 John Wiley \& Sons, Ltd.}, address = {Centre for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, DK-2800 Kgs. Lyngby, Denmark}, author = {Villas-B\^{o}as, Silas G. and H{\o}jer-Pedersen, Jesper and \r{A}kesson, Mats and Smedsgaard, J{\o}rn and Nielsen, Jens}, citeulike-article-id = {2393771}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/yea.1308}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/112130737/ABSTRACT}, doi = {10.1002/yea.1308}, journal = {Yeast}, keywords = {metabolomics, sampling, scerevisiae}, number = {14}, pages = {1155--1169}, posted-at = {2008-02-18 12:33:29}, priority = {2}, title = {Global metabolite analysis of yeast: evaluation of sample preparation methods}, url = {http://dx.doi.org/10.1002/yea.1308}, volume = {22}, year = {2005} }

TY - JOUR ID - citeulike:2393771 L3 - citeulike-article-id:2393771 N2 - Sample preparation is considered one of the limiting steps in microbial metabolome analysis. Eukaryotes and prokaryotes behave very differently during the several steps of classical sample preparation methods for analysis of metabolites. Even within the eukaryote kingdom there is a vast diversity of cell structures that make it imprudent to blindly adopt protocols that were designed for a specific group of microorganisms. We have therefore reviewed and evaluated the whole sample preparation procedures for analysis of yeast metabolites. Our focus has been on the current needs in metabolome analysis, which is the analysis of a large number of metabolites with very diverse chemical and physical properties. This work reports the leakage of intracellular metabolites observed during quenching yeast cells with cold methanol solution, the efficacy of six different methods for the extraction of intracellular metabolites, and the losses noticed during sample concentration by lyophilization and solvent evaporation. A more reliable procedure is suggested for quenching yeast cells with cold methanol solution, followed by extraction of intracellular metabolites by pure methanol. The method can be combined with reduced pressure solvent evaporation and therefore represents an attractive sample preparation procedure for high-throughput metabolome analysis of yeasts. Copyright © 2005 John Wiley & Sons, Ltd. IS - 14 JF - Yeast AD - Centre for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, DK-2800 Kgs. Lyngby, Denmark EP - 1169 TI - Global metabolite analysis of yeast: evaluation of sample preparation methods VL - 22 SP - 1155 KW - metabolomics KW - sampling KW - scerevisiae AU - Villas-Bôas, Silas AU - Højer-Pedersen, Jesper AU - Åkesson, Mats AU - Smedsgaard, Jørn AU - Nielsen, Jens PY - 2005/// UR - http://dx.doi.org/10.1002/yea.1308 ER -