Cold glycerol-saline: The promising quenching solution for accurate intracellular metabolite analysis of microbial cells Export

@article{citeulike:2393862, abstract = {Microbial metabolomics has been seriously limited by our inability to perform a reliable separation of intra- and extracellular metabolites with efficient quenching of cell metabolism. Microbial cells are sensitive to most (if not all) quenching agents developed to date, resulting in leakage of intracellular metabolites to the extracellular medium during quenching. Therefore, as yet we are unable to obtain an accurate concentration of intracellular metabolites from microbial cell cultures. However, knowledge of the in vivo concentrations of intermediary metabolites is of fundamental importance for the characterization of microbial metabolism so as to integrate meaningful metabolomics data with other levels of functional genomics analysis. In this article, we report a novel and robust quenching method for microbial cell cultures based on cold glycerol-saline solution as the quenching agent that prevents significant leakage of intracellular metabolites and, therefore, permits more accurate measurement of intracellular metabolite concentrations in microbial cells.}, author = {Villas-Boas, Silas G. and Bruheim, Per}, citeulike-article-id = {2393862}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ab.2007.06.028}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6W9V-4P1G9PD-3/2/b6aa8c08b1ed09accd3065f3533cf76c}, day = {1}, doi = {10.1016/j.ab.2007.06.028}, journal = {Analytical Biochemistry}, keywords = {metabolomics, sampling, scerevisiae}, month = {November}, number = {1}, pages = {87--97}, posted-at = {2008-02-18 12:51:39}, priority = {2}, title = {Cold glycerol-saline: The promising quenching solution for accurate intracellular metabolite analysis of microbial cells}, url = {http://dx.doi.org/10.1016/j.ab.2007.06.028}, volume = {370}, year = {2007} }

TY - JOUR ID - citeulike:2393862 L3 - citeulike-article-id:2393862 N2 - Microbial metabolomics has been seriously limited by our inability to perform a reliable separation of intra- and extracellular metabolites with efficient quenching of cell metabolism. Microbial cells are sensitive to most (if not all) quenching agents developed to date, resulting in leakage of intracellular metabolites to the extracellular medium during quenching. Therefore, as yet we are unable to obtain an accurate concentration of intracellular metabolites from microbial cell cultures. However, knowledge of the in vivo concentrations of intermediary metabolites is of fundamental importance for the characterization of microbial metabolism so as to integrate meaningful metabolomics data with other levels of functional genomics analysis. In this article, we report a novel and robust quenching method for microbial cell cultures based on cold glycerol-saline solution as the quenching agent that prevents significant leakage of intracellular metabolites and, therefore, permits more accurate measurement of intracellular metabolite concentrations in microbial cells. IS - 1 JF - Analytical Biochemistry EP - 97 TI - Cold glycerol-saline: The promising quenching solution for accurate intracellular metabolite analysis of microbial cells VL - 370 SP - 87 KW - metabolomics KW - sampling KW - scerevisiae AU - Villas-Boas, Silas AU - Bruheim, Per PY - 2007/11/01/ UR - http://dx.doi.org/10.1016/j.ab.2007.06.028 ER -