Transient increase of ATP as a response to temperature up-shift in Escherichia coli Export

@article{citeulike:2463859, abstract = {SUMMARY:BACKGROUND:Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for E. coli about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift.RESULTS:The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock.CONCLUSION:The presented data prove the very fast response of E. coli to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases.}, author = {Soini, Jaakko and Falschlehner, Christina and Mayer, Christina and Bohm, Daniela and Weinel, Stefan and Panula, Johanna and Vasala, Antti and Neubauer, Peter}, citeulike-article-id = {2463859}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/1475-2859-4-9}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15804347}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15804347}, doi = {10.1186/1475-2859-4-9}, journal = {Microbial Cell Factories}, keywords = {axp, temperature}, number = {1}, posted-at = {2008-03-04 10:33:07}, priority = {2}, title = {Transient increase of ATP as a response to temperature up-shift in Escherichia coli}, url = {http://dx.doi.org/10.1186/1475-2859-4-9}, volume = {4}, year = {2005} }

TY - JOUR ID - citeulike:2463859 L3 - citeulike-article-id:2463859 N2 - SUMMARY:BACKGROUND:Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for E. coli about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift.RESULTS:The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock.CONCLUSION:The presented data prove the very fast response of E. coli to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases. IS - 1 JF - Microbial Cell Factories TI - Transient increase of ATP as a response to temperature up-shift in Escherichia coli VL - 4 KW - axp KW - temperature AU - Soini, Jaakko AU - Falschlehner, Christina AU - Mayer, Christina AU - Bohm, Daniela AU - Weinel, Stefan AU - Panula, Johanna AU - Vasala, Antti AU - Neubauer, Peter PY - 2005/// UR - http://dx.doi.org/10.1186/1475-2859-4-9 ER -