In vivo analysis of metabolic dynamics in <I>Saccharomyces cerevisiae</I> : I. Experimental observations Export

@article{citeulike:2463869, abstract = {The goal of this work was to obtain rapid sampling technique to measure transient metabolites in vivo. First, a pulse of glucose was added to a culture of the yeast Saccharomyces cerevisiae growing aerobically under glucose limitation. Next, samples were removed at 2 to 5 s intervals and quenched using methods that depend on the metabolite measured. Extracellular glucose, excreted products, as well as glycolytic intermediates (G6P, F6P, FBP, GAP, 3-PG, PEP, Pyr) and cometabolites (ATP, ADP, AMP, NAD+, NADH) were measured using enzymatic or HPLC methods. Significant differences between the adenine nucleotide concentrations in the cytoplasm and mitochondria indicated the importance of compartmentation for the regulation of the glycolysis. Changes in the intra- and extracellular levels of metabolites confirmed that glycolysis is regulated on a time scale of seconds. {\copyright} 1997 John Wiley \& Sons, Inc. Biotechnol Bioeng 55: 305-316, 1997.}, address = {Institut f\"{u}r Bioverfahrenstechnik, Universit\"{a}t Stuttgart, Allmandring 31, 70569 Stuttgart, Germany; telephone: (49-711) 685-4573; fax: (49-711) 685-5164-stuttgart.de}, author = {Theobald, Uwe and Mailinger, Werner and Baltes, Michael and Rizzi, Manfred and Reuss, Matthias}, citeulike-article-id = {2463869}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/(SICI)1097-0290(19970720)55:2%3C305::AID-BIT8%3E3.0.CO;2-M}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/71003950/ABSTRACT}, doi = {10.1002/(SICI)1097-0290(19970720)55:2%3C305::AID-BIT8%3E3.0.CO;2-M}, journal = {Biotechnology and Bioengineering}, keywords = {axp, sampling}, number = {2}, pages = {305--316}, posted-at = {2008-03-04 10:40:41}, priority = {2}, title = {In vivo analysis of metabolic dynamics in <I>Saccharomyces cerevisiae</I> : I. Experimental observations}, url = {http://dx.doi.org/10.1002/(SICI)1097-0290(19970720)55:2%3C305::AID-BIT8%3E3.0.CO;2-M}, volume = {55}, year = {1997} }

TY - JOUR ID - citeulike:2463869 L3 - citeulike-article-id:2463869 N2 - The goal of this work was to obtain rapid sampling technique to measure transient metabolites in vivo. First, a pulse of glucose was added to a culture of the yeast Saccharomyces cerevisiae growing aerobically under glucose limitation. Next, samples were removed at 2 to 5 s intervals and quenched using methods that depend on the metabolite measured. Extracellular glucose, excreted products, as well as glycolytic intermediates (G6P, F6P, FBP, GAP, 3-PG, PEP, Pyr) and cometabolites (ATP, ADP, AMP, NAD+, NADH) were measured using enzymatic or HPLC methods. Significant differences between the adenine nucleotide concentrations in the cytoplasm and mitochondria indicated the importance of compartmentation for the regulation of the glycolysis. Changes in the intra- and extracellular levels of metabolites confirmed that glycolysis is regulated on a time scale of seconds. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 305-316, 1997. IS - 2 JF - Biotechnology and Bioengineering AD - Institut für Bioverfahrenstechnik, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany; telephone: (49-711) 685-4573; fax: (49-711) 685-5164-stuttgart.de EP - 316 TI - In vivo analysis of metabolic dynamics in <I>Saccharomyces cerevisiae</I> : I. Experimental observations VL - 55 SP - 305 KW - axp KW - sampling AU - Theobald, Uwe AU - Mailinger, Werner AU - Baltes, Michael AU - Rizzi, Manfred AU - Reuss, Matthias PY - 1997/// UR - http://dx.doi.org/10.1002/(SICI)1097-0290(19970720)55:2%3C305::AID-BIT8%3E3.0.CO;2-M ER -