Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation. Export

@article{citeulike:2911464, abstract = {To identify proteins that may participate in the activation of the protein kinase Raf, proteins that interact with Raf were selected in a two-hybrid screen. Two members of the 14-3-3 protein family were isolated that interacted with both the amino terminal regulatory regions of Raf and the kinase domain of Raf, but did not compete with the guanine nucleotide-binding protein Ras for binding to Raf. 14-3-3 proteins associated with Raf in mammalian cells and accompanied Raf to the membrane in the presence of activated Ras. In yeast cells expressing Raf and MEK, mammalian 14-3-3 beta or 14-3-3 zeta activated Raf to a similar extent as did expression of Ras. Therefore, 14-3-3 proteins may participate in or be required for the regulation of Raf function. These findings suggest a role for 14-3-3 proteins in Raf-mediated signal transduction.}, address = {Onyx Pharmaceuticals, Richmond, CA 94806-5206.}, author = {Freed, E. and Symons, M. and Macdonald, S. G. and McCormick, F. and Ruggieri, R.}, citeulike-article-id = {2911464}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8085158}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8085158}, day = {16}, issn = {0036-8075}, journal = {Science (New York, N.Y.)}, keywords = {14-3-3, signaling}, month = {September}, number = {5179}, pages = {1713--1716}, posted-at = {2008-06-20 21:44:32}, priority = {2}, title = {Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8085158}, volume = {265}, year = {1994} }

TY - JOUR ID - citeulike:2911464 L3 - citeulike-article-id:2911464 N2 - To identify proteins that may participate in the activation of the protein kinase Raf, proteins that interact with Raf were selected in a two-hybrid screen. Two members of the 14-3-3 protein family were isolated that interacted with both the amino terminal regulatory regions of Raf and the kinase domain of Raf, but did not compete with the guanine nucleotide-binding protein Ras for binding to Raf. 14-3-3 proteins associated with Raf in mammalian cells and accompanied Raf to the membrane in the presence of activated Ras. In yeast cells expressing Raf and MEK, mammalian 14-3-3 beta or 14-3-3 zeta activated Raf to a similar extent as did expression of Ras. Therefore, 14-3-3 proteins may participate in or be required for the regulation of Raf function. These findings suggest a role for 14-3-3 proteins in Raf-mediated signal transduction. IS - 5179 JF - Science (New York, N.Y.) SN - 0036-8075 AD - Onyx Pharmaceuticals, Richmond, CA 94806-5206. EP - 1716 TI - Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation. VL - 265 SP - 1713 KW - 14-3-3 KW - signaling AU - Freed, E AU - Symons, M AU - Macdonald, SG AU - McCormick, F AU - Ruggieri, R PY - 1994/09/16/ UR - http://view.ncbi.nlm.nih.gov/pubmed/8085158 ER -