Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes.

@article{citeulike:2964973, abstract = {Rab4 regulates recycling from early endosomes. We investigated the role of the rab4 effector rabaptin-5alpha and its putative partner gamma(1)-adaptin in membrane recycling. We found that rabaptin-5alpha forms a ternary complex with the gamma(1)-sigma(1) subcomplex of AP-1, via a direct interaction with the gamma(1)-subunit. The binding site for gamma(1)-adaptin is in the hinge region of rabaptin-5alpha, which is distinct from rab4- and rab5-binding domains. Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. Co-expressed rabaptin-5alpha and gamma(1)-adaptin, however, co-localized in a rab4-dependent manner on recycling endosomes. Transfection of rabaptin-5alpha caused enlarged endosomes and delayed recycling of transferrin. RNAi of rab4 had an opposing effect on transferrin recycling. Collectively, our data show that rab4-GTP acts as a scaffold for a rabaptin-5alpha- gamma(1)-adaptin complex on recycling endosomes and that interactions between rab4, rabaptin-5alpha and gamma(1)-adaptin regulate membrane recycling.}, address = {Department of Cell Biology and Institute of Biomembranes, University Medical Center, NL-3584 CX Utrecht, The Netherlands.}, author = {Deneka, M. and Neeft, M. and Popa, I. and van Oort, M. and Sprong, H. and Oorschot, V. and Klumperman, J. and Schu, P. and van der Sluijs, P.}, citeulike-article-id = {2964973}, issn = {0261-4189}, journal = {The EMBO journal}, keywords = {effector, rab-5, recycling}, month = {June}, number = {11}, pages = {2645--2657}, posted-at = {2008-07-04 20:45:49}, priority = {2}, title = {Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12773381}, volume = {22}, year = {2003} }

TY - JOUR ID - citeulike:2964973 L3 - citeulike-article-id:2964973 N2 - Rab4 regulates recycling from early endosomes. We investigated the role of the rab4 effector rabaptin-5alpha and its putative partner gamma(1)-adaptin in membrane recycling. We found that rabaptin-5alpha forms a ternary complex with the gamma(1)-sigma(1) subcomplex of AP-1, via a direct interaction with the gamma(1)-subunit. The binding site for gamma(1)-adaptin is in the hinge region of rabaptin-5alpha, which is distinct from rab4- and rab5-binding domains. Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. Co-expressed rabaptin-5alpha and gamma(1)-adaptin, however, co-localized in a rab4-dependent manner on recycling endosomes. Transfection of rabaptin-5alpha caused enlarged endosomes and delayed recycling of transferrin. RNAi of rab4 had an opposing effect on transferrin recycling. Collectively, our data show that rab4-GTP acts as a scaffold for a rabaptin-5alpha- gamma(1)-adaptin complex on recycling endosomes and that interactions between rab4, rabaptin-5alpha and gamma(1)-adaptin regulate membrane recycling. IS - 11 JF - The EMBO journal SN - 0261-4189 AD - Department of Cell Biology and Institute of Biomembranes, University Medical Center, NL-3584 CX Utrecht, The Netherlands. EP - 2657 TI - Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes. VL - 22 SP - 2645 KW - effector KW - rab-5 KW - recycling AU - Deneka, M AU - Neeft, M AU - Popa, I AU - van Oort, M AU - Sprong, H AU - Oorschot, V AU - Klumperman, J AU - Schu, P AU - van der Sluijs, P PY - 2003/06/02/ UR - http://view.ncbi.nlm.nih.gov/pubmed/12773381 ER -