Studies on Calcium Dependence Reveal Multiple Modes of Action for Triptolide Export

@article{citeulike:1002876, abstract = {SummaryTriptolide, a diterpene triepoxide isolated from the traditional Chinese medicinal vine Trypterygium wilfordii hook f., has been shown to induce rapid apoptosis in a myriad of cancer cell lines and inhibit NF[kappa]B transactivation. To understand further the general cellular mechanisms for this therapeutically relevant natural product, binding and biological activities were assessed. Studies showed that triptolide binding was saturable, reversible, and primarily localized to cell membranes. Depletion of calcium enhanced overall binding while differentially modulating biological function. Furthermore, triptolide's structural moieties demonstrated variability in the regulation of cell death versus inhibition of NF[kappa]B transactivation. These results implicate triptolide in the manipulation of at least two distinct cellular pathways with differing requirements for calcium and effective triptolide concentration in order to elicit each particular biological function.}, author = {Leuenroth, Stephanie J. and Crews, Craig M.}, citeulike-article-id = {1002876}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.chembiol.2005.09.009}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6VRP-4HTJYT7-4/2/82cec5c130e0f700cbb9340d767584a0}, doi = {10.1016/j.chembiol.2005.09.009}, journal = {Chemistry \& Biology}, keywords = {cell, locialise, membrane, on}, month = {December}, number = {12}, pages = {1259--1268}, posted-at = {2006-12-20 03:47:21}, priority = {3}, title = {Studies on Calcium Dependence Reveal Multiple Modes of Action for Triptolide}, url = {http://dx.doi.org/10.1016/j.chembiol.2005.09.009}, volume = {12}, year = {2005} }

TY - JOUR ID - citeulike:1002876 L3 - citeulike-article-id:1002876 N2 - SummaryTriptolide, a diterpene triepoxide isolated from the traditional Chinese medicinal vine Trypterygium wilfordii hook f., has been shown to induce rapid apoptosis in a myriad of cancer cell lines and inhibit NF[kappa]B transactivation. To understand further the general cellular mechanisms for this therapeutically relevant natural product, binding and biological activities were assessed. Studies showed that triptolide binding was saturable, reversible, and primarily localized to cell membranes. Depletion of calcium enhanced overall binding while differentially modulating biological function. Furthermore, triptolide's structural moieties demonstrated variability in the regulation of cell death versus inhibition of NF[kappa]B transactivation. These results implicate triptolide in the manipulation of at least two distinct cellular pathways with differing requirements for calcium and effective triptolide concentration in order to elicit each particular biological function. IS - 12 JF - Chemistry & Biology EP - 1268 TI - Studies on Calcium Dependence Reveal Multiple Modes of Action for Triptolide VL - 12 SP - 1259 KW - cell KW - locialise KW - membrane KW - on AU - Leuenroth, Stephanie AU - Crews, Craig PY - 2005/12// UR - http://dx.doi.org/10.1016/j.chembiol.2005.09.009 ER -