Determination of triptolide and triptonide in human plasma by high-performance liquid chromatography Export

@article{citeulike:1005542, abstract = {An isocratic high-performance liquid chromatographic method for determination of triptolide and triptonide in human plasma is described. Plasma samples were extracted with Oasis®HLB solid-phase extraction (SPE) cartridges. After pretreatment, they were separated on a SymmetryShieldTMRP18 column with a mobile phase of acetonitrile-water (40:60,v/v) at 40°C. The effluent was monitored at UV 217 nm. Linearity (0.010-1.0 mg/L) was good, and the lower limit of detection was 3 ng/mL for triptolide and 4.5 ng/mL for triptonide (S/N = 3). The relative standard deviations of intra- and inter-day assay were less than 15\% and the recoveries were better than 80\%. The developed method was applied to the determination of triptolide and triptonide concentration in a patient's plasma after taking the medicament containing Tripterygium wilfordii Hook. F. Copyright {\copyright} 2004 John Wiley \& Sons, Ltd.}, address = {The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road 38, Beijing 100083, People's Republic of China; Analytical Toxicology Department of Forensic Science Institute, Public Security Ministry, Beijing 3817 mail box 100038, People's Republic of China; Institute for Drug and Instrument Control, Health Department of GLD, PLA, Beijing 100071, People's Republic of China}, author = {Wang, Zhaohong and Wen, Jiao and Xing, Junbo and Guo, Dean}, citeulike-article-id = {1005542}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/bmc.416}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/109618617/ABSTRACT}, doi = {10.1002/bmc.416}, journal = {Biomedical Chromatography}, keywords = {hplc, in, plasma}, number = {1}, pages = {60--62}, posted-at = {2006-12-21 07:10:24}, priority = {0}, title = {Determination of triptolide and triptonide in human plasma by high-performance liquid chromatography}, url = {http://dx.doi.org/10.1002/bmc.416}, volume = {19}, year = {2005} }

TY - JOUR ID - citeulike:1005542 L3 - citeulike-article-id:1005542 N2 - An isocratic high-performance liquid chromatographic method for determination of triptolide and triptonide in human plasma is described. Plasma samples were extracted with Oasis®HLB solid-phase extraction (SPE) cartridges. After pretreatment, they were separated on a SymmetryShieldTMRP18 column with a mobile phase of acetonitrile-water (40:60,v/v) at 40°C. The effluent was monitored at UV 217 nm. Linearity (0.010-1.0 mg/L) was good, and the lower limit of detection was 3 ng/mL for triptolide and 4.5 ng/mL for triptonide (S/N = 3). The relative standard deviations of intra- and inter-day assay were less than 15% and the recoveries were better than 80%. The developed method was applied to the determination of triptolide and triptonide concentration in a patient's plasma after taking the medicament containing Tripterygium wilfordii Hook. F. Copyright © 2004 John Wiley & Sons, Ltd. IS - 1 JF - Biomedical Chromatography AD - The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road 38, Beijing 100083, People's Republic of China; Analytical Toxicology Department of Forensic Science Institute, Public Security Ministry, Beijing 3817 mail box 100038, People's Republic of China; Institute for Drug and Instrument Control, Health Department of GLD, PLA, Beijing 100071, People's Republic of China EP - 62 TI - Determination of triptolide and triptonide in human plasma by high-performance liquid chromatography VL - 19 SP - 60 KW - hplc KW - in KW - plasma AU - Wang, Zhaohong AU - Wen, Jiao AU - Xing, Junbo AU - Guo, Dean PY - 2005/// UR - http://dx.doi.org/10.1002/bmc.416 ER -