RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner Export

@article{citeulike:3153739, abstract = {The 5' untranslated region (5'UTR) of the psbA mRNA (psbA encodes the PSII reaction center protein, D1) is a key site for RNA-protein interactions in the post-transcriptional regulation of gene expression. In this study, we mapped the major psbA mRNA 5'-terminus at -77 nt, and two minor termini clusters centered at -48 and -64 nt, upstream from the psbA translational start codon of Arabidopsis thaliana. RNA mobility shift, RNase protection and UV-crosslinking assays were used to characterize the interaction of chloroplast proteins with the RNA 5'UTR. RNA-protein interactions depended upon a thermolabile secondary structure and specific sequences in a 35 nt region of the 5'UTR, which were 80\% conserved with the psbA 5'UTRs from five other plants. Major and minor proteins of 43- and 30-kDa, respectively, were detected by UV-crosslinking to RNA. Oxidizing conditions abolished the association of the proteins with the 5'UTR, while RNA-binding activity was recovered upon incubation with a reductant. Based on these findings, we hypothesize that post-transcriptional regulation of psbA gene expression in chloroplasts of vascular plants involves redox-dependent interactions between specific sequences in the 5'UTR and 43- and 30-kDa RNA-binding proteins. 10.1093/pcp/pce142}, author = {Shen, Yanxin and Danon, Avihai and Christopher, David A.}, citeulike-article-id = {3153739}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/pcp/pce142}, citeulike-linkout-1 = {http://pcp.oxfordjournals.org/cgi/content/abstract/42/10/1071}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11673622}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11673622}, day = {1}, doi = {10.1093/pcp/pce142}, journal = {Plant Cell Physiol.}, keywords = {5utr, interact, protein}, month = {October}, number = {10}, pages = {1071--1078}, posted-at = {2008-08-25 14:24:00}, priority = {4}, title = {RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner}, url = {http://dx.doi.org/10.1093/pcp/pce142}, volume = {42}, year = {2001} }

TY - JOUR ID - citeulike:3153739 L3 - citeulike-article-id:3153739 N2 - The 5' untranslated region (5'UTR) of the psbA mRNA (psbA encodes the PSII reaction center protein, D1) is a key site for RNA-protein interactions in the post-transcriptional regulation of gene expression. In this study, we mapped the major psbA mRNA 5'-terminus at -77 nt, and two minor termini clusters centered at -48 and -64 nt, upstream from the psbA translational start codon of Arabidopsis thaliana. RNA mobility shift, RNase protection and UV-crosslinking assays were used to characterize the interaction of chloroplast proteins with the RNA 5'UTR. RNA-protein interactions depended upon a thermolabile secondary structure and specific sequences in a 35 nt region of the 5'UTR, which were 80% conserved with the psbA 5'UTRs from five other plants. Major and minor proteins of 43- and 30-kDa, respectively, were detected by UV-crosslinking to RNA. Oxidizing conditions abolished the association of the proteins with the 5'UTR, while RNA-binding activity was recovered upon incubation with a reductant. Based on these findings, we hypothesize that post-transcriptional regulation of psbA gene expression in chloroplasts of vascular plants involves redox-dependent interactions between specific sequences in the 5'UTR and 43- and 30-kDa RNA-binding proteins. 10.1093/pcp/pce142 IS - 10 JF - Plant Cell Physiol. EP - 1078 TI - RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner VL - 42 SP - 1071 KW - 5utr KW - interact KW - protein AU - Shen, Yanxin AU - Danon, Avihai AU - Christopher, David PY - 2001/10/01/ UR - http://dx.doi.org/10.1093/pcp/pce142 ER -