Poly(A) polymerase activity and RNA polyadenylation in Streptomyces coelicolor A3(2) Export

@article{citeulike:694969, abstract = {The Streptomyces coelicolor genome sequence was searched for open reading frames (ORFs) similar to Escherichia coli poly(A) polymerase I, revealing an ORF with 36\% amino acid sequence identity to that protein. Mycelial extracts prepared from S. coelicolor cultures incorporated radioactive ATP into an acid-insoluble form, and some of the products of this incorporation had the properties expected of poly(A). [3H]-uridine and [3H]-adenosine were used to label the RNA in S. coelicolor cultures of different ages, and total RNA was fractionated by oligo dT cellulose chromatography. Approximately 3\% of the total uridine-labelled RNA and 11\% of the adenosine-labelled RNA were retained by the oligo dT cellulose columns. Enzymatic digestion of the retained RNA supported the conclusion that a significant fraction of the adenosine label was present in 3'-poly(A) chains. Measurement of poly(A) tail lengths by end labelling of total RNA and RNase digestion revealed a maximum length of = 18 residues. Radioactive cDNA prepared from the RNA fraction retained by oligo dT cellulose hybridized to the 16S and 23S genes from a streptomycete ribosomal RNA operon but not to the 5S gene. Reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of mRNAs in the RNA fraction retained by oligo dT cellulose.}, author = {Bralley, Patricia and Jones, George H.}, citeulike-article-id = {694969}, citeulike-linkout-0 = {http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2001.02457.x}, citeulike-linkout-1 = {http://dx.doi.org/10.1046/j.1365-2958.2001.02457.x}, doi = {10.1046/j.1365-2958.2001.02457.x}, journal = {Molecular Microbiology}, keywords = {bacteria, mrna, polya}, number = {5}, pages = {1155--1164}, posted-at = {2006-06-13 16:39:01}, priority = {2}, title = {Poly(A) polymerase activity and RNA polyadenylation in Streptomyces coelicolor A3(2)}, url = {http://dx.doi.org/10.1046/j.1365-2958.2001.02457.x}, volume = {40}, year = {2001} }

TY - JOUR ID - citeulike:694969 L3 - citeulike-article-id:694969 N2 - The Streptomyces coelicolor genome sequence was searched for open reading frames (ORFs) similar to Escherichia coli poly(A) polymerase I, revealing an ORF with 36% amino acid sequence identity to that protein. Mycelial extracts prepared from S. coelicolor cultures incorporated radioactive ATP into an acid-insoluble form, and some of the products of this incorporation had the properties expected of poly(A). [3H]-uridine and [3H]-adenosine were used to label the RNA in S. coelicolor cultures of different ages, and total RNA was fractionated by oligo dT cellulose chromatography. Approximately 3% of the total uridine-labelled RNA and 11% of the adenosine-labelled RNA were retained by the oligo dT cellulose columns. Enzymatic digestion of the retained RNA supported the conclusion that a significant fraction of the adenosine label was present in 3'-poly(A) chains. Measurement of poly(A) tail lengths by end labelling of total RNA and RNase digestion revealed a maximum length of = 18 residues. Radioactive cDNA prepared from the RNA fraction retained by oligo dT cellulose hybridized to the 16S and 23S genes from a streptomycete ribosomal RNA operon but not to the 5S gene. Reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of mRNAs in the RNA fraction retained by oligo dT cellulose. IS - 5 JF - Molecular Microbiology EP - 1164 TI - Poly(A) polymerase activity and RNA polyadenylation in Streptomyces coelicolor A3(2) VL - 40 SP - 1155 KW - bacteria KW - mrna KW - polya AU - Bralley, Patricia AU - Jones, George PY - 2001/// UR - http://dx.doi.org/10.1046/j.1365-2958.2001.02457.x ER -