Bacteriophage T7 mRNA is polyadenylated Export

@article{citeulike:695132, abstract = {To determine whether the RNA of bacterial viruses is polyadenylated like bacterial mRNAs, pulse-labelled as well as the steady-state population of bacteriophage T7-specific transcripts were examined for the presence of poly(A) tracts by binding to oligo(dT) cellulose followed by hybridization with specific gene probes. Representatives of all classes of bacteriophage-specific mRNA - early, middle and late - were found to be polyadenylated. This conclusion was confirmed by screening the products of oligo(dT)-dependent cDNA synthesis. A cDNA library was prepared from RNA synthesized after bacteriophage T7 infection and the sequence of bacteriophage-specific clones was determined to define the sites of polyadenylation. About half of the clones were polyadenylated near the end of a protein-coding region, one of them at the site of post-transcriptional processing by RNase III. Other clones were polyadenylated within protein-coding regions. These observations suggest that polyadenylation occurs after the nucleolytic processing of primary transcripts and in some cases also after mRNA degradation has already begun.}, author = {Johnson, M. D. and Popowski, J. and Cao, G. J. and Shen, P. and Sarkar, N.}, citeulike-article-id = {695132}, citeulike-linkout-0 = {http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.1998.00649.x}, citeulike-linkout-1 = {http://dx.doi.org/10.1046/j.1365-2958.1998.00649.x}, doi = {10.1046/j.1365-2958.1998.00649.x}, journal = {Molecular Microbiology}, number = {1}, pages = {23--30}, posted-at = {2006-06-13 19:47:29}, priority = {2}, title = {Bacteriophage T7 mRNA is polyadenylated}, url = {http://dx.doi.org/10.1046/j.1365-2958.1998.00649.x}, volume = {27}, year = {1998} }

TY - JOUR ID - citeulike:695132 L3 - citeulike-article-id:695132 N2 - To determine whether the RNA of bacterial viruses is polyadenylated like bacterial mRNAs, pulse-labelled as well as the steady-state population of bacteriophage T7-specific transcripts were examined for the presence of poly(A) tracts by binding to oligo(dT) cellulose followed by hybridization with specific gene probes. Representatives of all classes of bacteriophage-specific mRNA - early, middle and late - were found to be polyadenylated. This conclusion was confirmed by screening the products of oligo(dT)-dependent cDNA synthesis. A cDNA library was prepared from RNA synthesized after bacteriophage T7 infection and the sequence of bacteriophage-specific clones was determined to define the sites of polyadenylation. About half of the clones were polyadenylated near the end of a protein-coding region, one of them at the site of post-transcriptional processing by RNase III. Other clones were polyadenylated within protein-coding regions. These observations suggest that polyadenylation occurs after the nucleolytic processing of primary transcripts and in some cases also after mRNA degradation has already begun. IS - 1 JF - Molecular Microbiology EP - 30 TI - Bacteriophage T7 mRNA is polyadenylated VL - 27 SP - 23 AU - Johnson, MD AU - Popowski, J AU - Cao, GJ AU - Shen, P AU - Sarkar, N PY - 1998/// UR - http://dx.doi.org/10.1046/j.1365-2958.1998.00649.x ER -