Effects of legacy nuclear waste on the compositional diversity and distributions of sulfate-reducing bacteria in a terrestrial subsurface aquifer Export

@article{citeulike:702912, abstract = {Abstract The impact of legacy nuclear waste on the compositional diversity and distribution of sulfate-reducing bacteria in a heavily contaminated subsurface aquifer was examined. dsrAB clone libraries were constructed and restriction fragment length polymorphism (RFLP) analysis used to evaluate genetic variation between sampling wells. Principal component analysis identified nickel, nitrate, technetium, and organic carbon as the primary variables contributing to well-to-well geochemical variability, although comparative sequence analysis showed the sulfate-reducing bacteria community structure to be consistent throughout contaminated and uncontaminated regions of the aquifer. Only 3\% of recovered dsrAB gene sequences showed apparent membership to the Deltaproteobacteria. The remainder of recovered sequences may represent novel, deep-branching lineages that, to our knowledge, do not presently contain any cultivated members; although corresponding phylotypes have recently been reported from several different marine ecosystems. These findings imply resiliency and adaptability of sulfate-reducing bacteria to extremes in environmental conditions, although the possibility for horizontal transfer of dsrAB is also discussed.}, author = {Bagwell, Christopher E. and Liu, Xuaduan and Wu, Liyou and Zhou, Jizhong}, citeulike-article-id = {702912}, citeulike-linkout-0 = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6941.2005.00039.x}, citeulike-linkout-1 = {http://dx.doi.org/10.1111/j.1574-6941.2005.00039.x}, doi = {10.1111/j.1574-6941.2005.00039.x}, journal = {FEMS Microbiology Ecology}, keywords = {ecology, j, microbial, zhou}, number = {3}, pages = {424--431}, posted-at = {2006-06-20 14:45:03}, priority = {2}, title = {Effects of legacy nuclear waste on the compositional diversity and distributions of sulfate-reducing bacteria in a terrestrial subsurface aquifer}, url = {http://dx.doi.org/10.1111/j.1574-6941.2005.00039.x}, volume = {55}, year = {2006} }

TY - JOUR ID - citeulike:702912 L3 - citeulike-article-id:702912 N2 - Abstract The impact of legacy nuclear waste on the compositional diversity and distribution of sulfate-reducing bacteria in a heavily contaminated subsurface aquifer was examined. dsrAB clone libraries were constructed and restriction fragment length polymorphism (RFLP) analysis used to evaluate genetic variation between sampling wells. Principal component analysis identified nickel, nitrate, technetium, and organic carbon as the primary variables contributing to well-to-well geochemical variability, although comparative sequence analysis showed the sulfate-reducing bacteria community structure to be consistent throughout contaminated and uncontaminated regions of the aquifer. Only 3% of recovered dsrAB gene sequences showed apparent membership to the Deltaproteobacteria. The remainder of recovered sequences may represent novel, deep-branching lineages that, to our knowledge, do not presently contain any cultivated members; although corresponding phylotypes have recently been reported from several different marine ecosystems. These findings imply resiliency and adaptability of sulfate-reducing bacteria to extremes in environmental conditions, although the possibility for horizontal transfer of dsrAB is also discussed. IS - 3 JF - FEMS Microbiology Ecology EP - 431 TI - Effects of legacy nuclear waste on the compositional diversity and distributions of sulfate-reducing bacteria in a terrestrial subsurface aquifer VL - 55 SP - 424 KW - ecology KW - j KW - microbial KW - zhou AU - Bagwell, Christopher AU - Liu, Xuaduan AU - Wu, Liyou AU - Zhou, Jizhong PY - 2006/// UR - http://dx.doi.org/10.1111/j.1574-6941.2005.00039.x ER -