Production of Heterologous and Chimeric Scaffoldins by Clostridium acetobutylicum ATCC 824 Export

@article{citeulike:703099, abstract = {Clostridium acetobutylicum ATCC 824 converts sugars and various polysaccharides into acids and solvents. This bacterium, however, is unable to utilize cellulosic substrates, since it is able to secrete very small amounts of cellulosomes. To promote the utilization of crystalline cellulose, the strategy we chose aims at producing heterologous minicellulosomes, containing two different cellulases bound to a miniscaffoldin, in C. acetobutylicum. A first step toward this goal describes the production of miniCipC1, a truncated form of CipC from Clostridium cellulolyticum, and the hybrid scaffoldin Scaf 3, which bears an additional cohesin domain derived from CipA from Clostridium thermocellum. Both proteins were correctly matured and secreted in the medium, and their various domains were found to be functional.}, author = {Perret, S. and Casalot, L. and Fierobe, H. P. and Tardif, C. and Sabathe, F. and Belaich, J. P. and Belaich, A.}, citeulike-article-id = {703099}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JB.186.1.253}, citeulike-linkout-1 = {http://jb.asm.org/cgi/content/abstract/186/1/253}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14679247}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14679247}, day = {1}, doi = {10.1128/JB.186.1.253}, journal = {J. Bacteriol.}, keywords = {clostridia, engineering}, month = {January}, number = {1}, pages = {253--257}, posted-at = {2006-06-20 15:30:08}, priority = {2}, title = {Production of Heterologous and Chimeric Scaffoldins by Clostridium acetobutylicum ATCC 824}, url = {http://dx.doi.org/10.1128/JB.186.1.253}, volume = {186}, year = {2004} }

TY - JOUR ID - citeulike:703099 L3 - citeulike-article-id:703099 N2 - Clostridium acetobutylicum ATCC 824 converts sugars and various polysaccharides into acids and solvents. This bacterium, however, is unable to utilize cellulosic substrates, since it is able to secrete very small amounts of cellulosomes. To promote the utilization of crystalline cellulose, the strategy we chose aims at producing heterologous minicellulosomes, containing two different cellulases bound to a miniscaffoldin, in C. acetobutylicum. A first step toward this goal describes the production of miniCipC1, a truncated form of CipC from Clostridium cellulolyticum, and the hybrid scaffoldin Scaf 3, which bears an additional cohesin domain derived from CipA from Clostridium thermocellum. Both proteins were correctly matured and secreted in the medium, and their various domains were found to be functional. IS - 1 JF - J. Bacteriol. EP - 257 TI - Production of Heterologous and Chimeric Scaffoldins by Clostridium acetobutylicum ATCC 824 VL - 186 SP - 253 KW - clostridia KW - engineering AU - Perret, S AU - Casalot, L AU - Fierobe, HP AU - Tardif, C AU - Sabathe, F AU - Belaich, JP AU - Belaich, A PY - 2004/01/01/ UR - http://dx.doi.org/10.1128/JB.186.1.253 ER -