Complexation of Uranium by Cells and S-Layer Sheets of Bacillus sphaericus JG-A12 Export

@article{citeulike:714046, abstract = {Bacillus sphaericus JG-A12 is a natural isolate recovered from a uranium mining waste pile near the town of Johanngeorgenstadt in Saxony, Germany. The cells of this strain are enveloped by a highly ordered crystalline proteinaceous surface layer (S-layer) possessing an ability to bind uranium and other heavy metals. Purified and recrystallized S-layer proteins were shown to be phosphorylated by phosphoprotein-specific staining, inductive coupled plasma mass spectrometry analysis, and a colorimetric method. We used extended X-ray absorption fine-structure (EXAFS) spectroscopy to determine the structural parameters of the uranium complexes formed by purified and recrystallized S-layer sheets of B. sphaericus JG-A12. In addition, we investigated the complexation of uranium by the vegetative bacterial cells. The EXAFS analysis demonstrated that in all samples studied, the U(VI) is coordinated to carboxyl groups in a bidentate fashion with an average distance between the U atom and the C atom of 2.88 {+/-} 0.02 A and to phosphate groups in a monodentate fashion with an average distance between the U atom and the P atom of 3.62 {+/-} 0.02 A. Transmission electron microscopy showed that the uranium accumulated by the cells of this strain is located in dense deposits at the cell surface.}, author = {Merroun, Mohamed L. and Raff, Johannes and Rossberg, Andre and Hennig, Christoph and Reich, Tobias and Selenska-Pobell, Sonja}, citeulike-article-id = {714046}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.71.9.5532}, citeulike-linkout-1 = {http://aem.asm.org/cgi/content/abstract/71/9/5532}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16151146}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16151146}, day = {1}, doi = {10.1128/AEM.71.9.5532}, journal = {Appl. Environ. Microbiol.}, keywords = {accumulation, bacteria, by, uranium}, month = {September}, number = {9}, pages = {5532--5543}, posted-at = {2006-06-28 16:36:17}, priority = {2}, title = {Complexation of Uranium by Cells and S-Layer Sheets of Bacillus sphaericus JG-A12}, url = {http://dx.doi.org/10.1128/AEM.71.9.5532}, volume = {71}, year = {2005} }

TY - JOUR ID - citeulike:714046 L3 - citeulike-article-id:714046 N2 - Bacillus sphaericus JG-A12 is a natural isolate recovered from a uranium mining waste pile near the town of Johanngeorgenstadt in Saxony, Germany. The cells of this strain are enveloped by a highly ordered crystalline proteinaceous surface layer (S-layer) possessing an ability to bind uranium and other heavy metals. Purified and recrystallized S-layer proteins were shown to be phosphorylated by phosphoprotein-specific staining, inductive coupled plasma mass spectrometry analysis, and a colorimetric method. We used extended X-ray absorption fine-structure (EXAFS) spectroscopy to determine the structural parameters of the uranium complexes formed by purified and recrystallized S-layer sheets of B. sphaericus JG-A12. In addition, we investigated the complexation of uranium by the vegetative bacterial cells. The EXAFS analysis demonstrated that in all samples studied, the U(VI) is coordinated to carboxyl groups in a bidentate fashion with an average distance between the U atom and the C atom of 2.88 {+/-} 0.02 A and to phosphate groups in a monodentate fashion with an average distance between the U atom and the P atom of 3.62 {+/-} 0.02 A. Transmission electron microscopy showed that the uranium accumulated by the cells of this strain is located in dense deposits at the cell surface. IS - 9 JF - Appl. Environ. Microbiol. EP - 5543 TI - Complexation of Uranium by Cells and S-Layer Sheets of Bacillus sphaericus JG-A12 VL - 71 SP - 5532 KW - accumulation KW - bacteria KW - by KW - uranium AU - Merroun, Mohamed AU - Raff, Johannes AU - Rossberg, Andre AU - Hennig, Christoph AU - Reich, Tobias AU - Selenska-Pobell, Sonja PY - 2005/09/01/ UR - http://dx.doi.org/10.1128/AEM.71.9.5532 ER -