Mitochondrial Metabolism in Developing Embryos of Brassica napus Export

@article{citeulike:933712, abstract = {The metabolism of developing plant seeds is directed toward transforming primary assimilatory products (sugars and amino acids) into seed storage compounds. To understand the role of mitochondria in this metabolism, metabolic fluxes were determined in developing embryos of Brassica napus. After labeling with [1,2-13C2]glucose + [U-13C6]glucose, [U-13C3]alanine, [U-13C5]glutamine, [15N]alanine, (amino)-[15N]glutamine, or (amide)-[15N]glutamine, the resulting labeling patterns in protein amino acids and in fatty acids were analyzed by gas chromatography-mass spectrometry. Fluxes through mitochondrial metabolism were quantified using a steady state flux model. Labeling information from experiments using different labeled substrates was essential for model validation and reliable flux estimation. The resulting flux map shows that mitochondrial metabolism in these developing seeds is very different from that in either heterotrophic or autotrophic plant tissues or in most other organisms: (i) flux around the tricarboxylic acid cycle is absent and the small fluxes through oxidative reactions in the mitochondrion can generate (via oxidative phosphorylation) at most 22\% of the ATP needed for biosynthesis; (ii) isocitrate dehydrogenase is reversible in vivo; (iii) about 40\% of mitochondrial pyruvate is produced by malic enzyme rather than being imported from the cytosol; (iv) mitochondrial flux is largely devoted to providing precursors for cytosolic fatty acid elongation; and (v) the uptake of amino acids rather than anaplerosis via PEP carboxylase determines carbon flow into storage proteins. 10.1074/jbc.M606266200}, author = {Schwender, Jorg and Shachar-Hill, Yair and Ohlrogge, John B.}, citeulike-article-id = {933712}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M606266200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/281/45/34040}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16971389}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16971389}, day = {10}, doi = {10.1074/jbc.M606266200}, journal = {J. Biol. Chem.}, keywords = {flexes, metabolite}, month = {November}, number = {45}, pages = {34040--34047}, posted-at = {2006-11-06 17:02:07}, priority = {2}, title = {Mitochondrial Metabolism in Developing Embryos of Brassica napus}, url = {http://dx.doi.org/10.1074/jbc.M606266200}, volume = {281}, year = {2006} }

TY - JOUR ID - citeulike:933712 L3 - citeulike-article-id:933712 N2 - The metabolism of developing plant seeds is directed toward transforming primary assimilatory products (sugars and amino acids) into seed storage compounds. To understand the role of mitochondria in this metabolism, metabolic fluxes were determined in developing embryos of Brassica napus. After labeling with [1,2-13C2]glucose + [U-13C6]glucose, [U-13C3]alanine, [U-13C5]glutamine, [15N]alanine, (amino)-[15N]glutamine, or (amide)-[15N]glutamine, the resulting labeling patterns in protein amino acids and in fatty acids were analyzed by gas chromatography-mass spectrometry. Fluxes through mitochondrial metabolism were quantified using a steady state flux model. Labeling information from experiments using different labeled substrates was essential for model validation and reliable flux estimation. The resulting flux map shows that mitochondrial metabolism in these developing seeds is very different from that in either heterotrophic or autotrophic plant tissues or in most other organisms: (i) flux around the tricarboxylic acid cycle is absent and the small fluxes through oxidative reactions in the mitochondrion can generate (via oxidative phosphorylation) at most 22% of the ATP needed for biosynthesis; (ii) isocitrate dehydrogenase is reversible in vivo; (iii) about 40% of mitochondrial pyruvate is produced by malic enzyme rather than being imported from the cytosol; (iv) mitochondrial flux is largely devoted to providing precursors for cytosolic fatty acid elongation; and (v) the uptake of amino acids rather than anaplerosis via PEP carboxylase determines carbon flow into storage proteins. 10.1074/jbc.M606266200 IS - 45 JF - J. Biol. Chem. EP - 34047 TI - Mitochondrial Metabolism in Developing Embryos of Brassica napus VL - 281 SP - 34040 KW - flexes KW - metabolite AU - Schwender, Jorg AU - Shachar-Hill, Yair AU - Ohlrogge, John PY - 2006/11/10/ UR - http://dx.doi.org/10.1074/jbc.M606266200 ER -