Expression of beta(2)-adrenergic receptor in oral squamous cell carcinoma. Export

@article{citeulike:3908448, abstract = {J Oral Pathol Med (2008)Background: It has been speculated that chemokines and neurotransmitters might be involved in the organ-specific development of metastases because cancer metastasis is similar to the regulation of migratory activity in leukocytes. Here, we aimed to examine the expression of beta(2)-adrenergic receptor (beta(2)-AR) in oral squamous cell carcinoma (OSCC), and to investigate its correlation with tumor development and metastasis. Methods: Expression of beta(2)-AR was examined in 65 cases of OSCC specimens, 10 cases of normal oral mucosa, and two cell lines using immunohistochemistry, Western blot and RT-PCR. The differences in beta(2)-AR expression between various groups were evaluated using SPSS 13.0 Statistical Software. Cell proliferation assays were assayed by beta-adrenergic receptors agonists (norepinephrine) and antagonists (propranolol). Norepinephrine-mediated cell migration was assayed in Matrigel-coated chemotaxis chamber. Results: beta(2)-AR was highly expressed on OSCC compared to normal controls. In OSCC, positive beta(2)-AR expression was significantly correlated with cervical lymph node metastasis (P = 0.001), age (P = 0.003), tumor size (P = 0.001) and clinical stage (P = 0.001), but not with gender. RT-PCR and Western blot also confirmed positive beta(2)-AR expression in OSCC and TCa8113 cell line, and negative beta(2)-AR expression in normal oral mucosa and ACC cell line. beta-adrenoreceptor agonist (norepinephrine) was a potent mitogen for TCa8113 and ACC cell lines, and completely inhibited by the selective antagonist of beta-adrenergic receptors (propranolol). Norepinephrine induced migratory activity of OSCC cells in a dose-dependent manner. Conclusion: Increased expression of beta(2)-AR may play an important role in the formation and metastasis of OSCC.}, author = {Shang, Zheng Jun J. and Liu, Ke and Liang, De Feng F.}, citeulike-article-id = {3908448}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1600-0714.2008.00691.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19141064}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19141064}, day = {26}, doi = {10.1111/j.1600-0714.2008.00691.x}, issn = {1600-0714}, journal = {Journal of oral pathology \& medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology}, keywords = {b2ar, cancer, disease, expression}, month = {December}, posted-at = {2009-01-19 13:57:04}, priority = {2}, title = {Expression of beta(2)-adrenergic receptor in oral squamous cell carcinoma.}, url = {http://dx.doi.org/10.1111/j.1600-0714.2008.00691.x}, year = {2008} }

TY - JOUR ID - citeulike:3908448 L3 - citeulike-article-id:3908448 N2 - J Oral Pathol Med (2008)Background: It has been speculated that chemokines and neurotransmitters might be involved in the organ-specific development of metastases because cancer metastasis is similar to the regulation of migratory activity in leukocytes. Here, we aimed to examine the expression of beta(2)-adrenergic receptor (beta(2)-AR) in oral squamous cell carcinoma (OSCC), and to investigate its correlation with tumor development and metastasis. Methods: Expression of beta(2)-AR was examined in 65 cases of OSCC specimens, 10 cases of normal oral mucosa, and two cell lines using immunohistochemistry, Western blot and RT-PCR. The differences in beta(2)-AR expression between various groups were evaluated using SPSS 13.0 Statistical Software. Cell proliferation assays were assayed by beta-adrenergic receptors agonists (norepinephrine) and antagonists (propranolol). Norepinephrine-mediated cell migration was assayed in Matrigel-coated chemotaxis chamber. Results: beta(2)-AR was highly expressed on OSCC compared to normal controls. In OSCC, positive beta(2)-AR expression was significantly correlated with cervical lymph node metastasis (P = 0.001), age (P = 0.003), tumor size (P = 0.001) and clinical stage (P = 0.001), but not with gender. RT-PCR and Western blot also confirmed positive beta(2)-AR expression in OSCC and TCa8113 cell line, and negative beta(2)-AR expression in normal oral mucosa and ACC cell line. beta-adrenoreceptor agonist (norepinephrine) was a potent mitogen for TCa8113 and ACC cell lines, and completely inhibited by the selective antagonist of beta-adrenergic receptors (propranolol). Norepinephrine induced migratory activity of OSCC cells in a dose-dependent manner. Conclusion: Increased expression of beta(2)-AR may play an important role in the formation and metastasis of OSCC. JF - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology SN - 1600-0714 TI - Expression of beta(2)-adrenergic receptor in oral squamous cell carcinoma. KW - b2ar KW - cancer KW - disease KW - expression AU - Shang, Zheng Jun AU - Liu, Ke AU - Liang, De Feng PY - 2008/12/26/ UR - http://dx.doi.org/10.1111/j.1600-0714.2008.00691.x ER -