Modern Homology Modeling of G-Protein Coupled Receptors: Which Structural Template to Use? Export

@article{citeulike:5243260, abstract = {The recent availability in the literature of new crystal structures of inactive G-protein coupled receptors (GPCRs) prompted us to study the extent to which these crystal structures constitute an advantage over the former prototypic rhodopsin template for homology modeling of the transmembrane (TM) region of human class A GPCRs. Our results suggest that better templates than those currently available are required by the majority of these GPCRs to generate homology models that are accurate enough for simple virtual screening aimed at computer-aided drug discovery. Thus, we investigated: (1) which class A GPCRs would have the highest impact as potential templates for homology modeling of other GPCRs, if their structures were solved, and (2) the extent to which multiple-template homology modeling (using all currently available GPCR crystal structures) provides an improvement over single-template homology modeling, as evaluated by the accuracy of rigid protein−flexible ligand docking on these models.}, author = {Mobarec, Juan C. and Sanchez, Roberto and Filizola, Marta}, citeulike-article-id = {5243260}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/jm9005252}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/jm9005252}, day = {27}, doi = {10.1021/jm9005252}, journal = {Journal of Medicinal Chemistry}, keywords = {docking, gpcr, modeling, theory}, month = {August}, number = {16}, pages = {5207--5216}, posted-at = {2009-10-08 16:39:12}, priority = {2}, title = {Modern Homology Modeling of G-Protein Coupled Receptors: Which Structural Template to Use?}, url = {http://dx.doi.org/10.1021/jm9005252}, volume = {52}, year = {2009} }

TY - JOUR ID - citeulike:5243260 L3 - citeulike-article-id:5243260 N2 - The recent availability in the literature of new crystal structures of inactive G-protein coupled receptors (GPCRs) prompted us to study the extent to which these crystal structures constitute an advantage over the former prototypic rhodopsin template for homology modeling of the transmembrane (TM) region of human class A GPCRs. Our results suggest that better templates than those currently available are required by the majority of these GPCRs to generate homology models that are accurate enough for simple virtual screening aimed at computer-aided drug discovery. Thus, we investigated: (1) which class A GPCRs would have the highest impact as potential templates for homology modeling of other GPCRs, if their structures were solved, and (2) the extent to which multiple-template homology modeling (using all currently available GPCR crystal structures) provides an improvement over single-template homology modeling, as evaluated by the accuracy of rigid protein−flexible ligand docking on these models. IS - 16 JF - Journal of Medicinal Chemistry EP - 5216 TI - Modern Homology Modeling of G-Protein Coupled Receptors: Which Structural Template to Use? VL - 52 SP - 5207 KW - docking KW - gpcr KW - modeling KW - theory AU - Mobarec, Juan AU - Sanchez, Roberto AU - Filizola, Marta PY - 2009/08/27/ UR - http://dx.doi.org/10.1021/jm9005252 ER -