Regulation of transcript elongation through cooperative and ordered recruitment of cofactors Export

@article{citeulike:1346313, abstract = {We studied the regulation of murine CD80, a gene whose basal transcriptional status was characterized by the presence of a stalled RNA polymerase II complex on the promoter-proximal region. Stimulus-induced activation of productive elongation involved a complex interplay of regulated events that included a synergy between ordered co-factor recruitment. This cascade of recruitments was initiated through the engagement of transcription factor NF-[kappa]B, leading to the temporal association of histone acetyltransferases and the consequent selective acetylation of a transcription start site-downstream nucleosome. This in turn culminated into the nucleosomal association of Brd4-associated P-TEFb, a protein complex containing kinase specific for serine 2 of Rbp 1, the largest subunit of carboxyl terminal domain (CTD) of RNA polymerase II. The consequent phosphorylation of serine 2 residues in CTD by CDK9 in P-TEFb complex then facilitated escape of polymerase II into the productive elongation phase. Thus, the cooperative mechanisms that integrate between independent pathways characterize regulation of the elongation step of transcription; thereby providing another level at which specificity of gene regulation can be achieved. 10.1074/jbc.M701420200}, author = {Sharma, Manish and George, Anuja A. and Singh, Badri N. and Sahoo, Naresh C. and Rao, Kanury V.}, citeulike-article-id = {1346313}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M701420200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17535807}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17535807}, day = {29}, doi = {10.1074/jbc.M701420200}, journal = {J. Biol. Chem.}, keywords = {cofactor, cofactors, cooperative, elongation, mrna, recruitment, rnapii, transcript}, month = {May}, pages = {M701420200+}, posted-at = {2007-06-04 22:40:54}, priority = {2}, title = {Regulation of transcript elongation through cooperative and ordered recruitment of cofactors}, url = {http://dx.doi.org/10.1074/jbc.M701420200}, year = {2007} }

TY - JOUR ID - citeulike:1346313 L3 - citeulike-article-id:1346313 N2 - We studied the regulation of murine CD80, a gene whose basal transcriptional status was characterized by the presence of a stalled RNA polymerase II complex on the promoter-proximal region. Stimulus-induced activation of productive elongation involved a complex interplay of regulated events that included a synergy between ordered co-factor recruitment. This cascade of recruitments was initiated through the engagement of transcription factor NF-[kappa]B, leading to the temporal association of histone acetyltransferases and the consequent selective acetylation of a transcription start site-downstream nucleosome. This in turn culminated into the nucleosomal association of Brd4-associated P-TEFb, a protein complex containing kinase specific for serine 2 of Rbp 1, the largest subunit of carboxyl terminal domain (CTD) of RNA polymerase II. The consequent phosphorylation of serine 2 residues in CTD by CDK9 in P-TEFb complex then facilitated escape of polymerase II into the productive elongation phase. Thus, the cooperative mechanisms that integrate between independent pathways characterize regulation of the elongation step of transcription; thereby providing another level at which specificity of gene regulation can be achieved. 10.1074/jbc.M701420200 JF - J. Biol. Chem. TI - Regulation of transcript elongation through cooperative and ordered recruitment of cofactors SP - M701420200 KW - cofactor KW - cofactors KW - cooperative KW - elongation KW - mrna KW - recruitment KW - rnapii KW - transcript AU - Sharma, Manish AU - George, Anuja AU - Singh, Badri AU - Sahoo, Naresh AU - Rao, Kanury PY - 2007/05/29/ UR - http://dx.doi.org/10.1074/jbc.M701420200 ER -