The inheritance of chemical phenotype in Cannabis sativa L.

@article{citeulike:2835225, abstract = {Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F(1)'s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual F(1) plants were self-fertilized, and 10 inbred F(2) offspring were collected and analyzed. In all cases, a segregation of the three chemotypes (pure CBD, mixed CBD-THC, and pure THC) fitting a 1:2:1 proportion was observed. The CBD/THC ratio was found to be significantly progeny specific and transmitted from each F(1) to the F(2)'s derived from it. A model involving one locus, B, with two alleles, B(D) and B(T), is proposed, with the two alleles being codominant. The mixed chemotypes are interpreted as due to the genotype B(D)/B(T) at the B locus, while the pure-chemotype plants are due to homozygosity at the B locus (either B(D)/B(D) or B(T)/B(T)). It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC, respectively. The F(2) segregating groups were used in a bulk segregant analysis of the pooled DNAs for screening RAPD primers; three chemotype-associated markers are described, one of which has been transformed in a sequence-characterized amplified region (SCAR) marker and shows tight linkage to the chemotype and codominance.}, address = {HortaPharm B.V., 1075 VS, Amsterdam, The Netherlands.}, author = {de Meijer, E. P. and Bagatta, M. and Carboni, A. and Crucitti, P. and Moliterni, V. M. and Ranalli, P. and Mandolino, G. }, citeulike-article-id = {2835225}, issn = {0016-6731}, journal = {Genetics}, keywords = {cannabidiol, cannabinoid, cannabis, cdb, chemical, chemotype, codominance, cross, inheritance, locus, marker, phenotype, plant, rapd, sativa, scar, selfing, synthase, tetrahydrocannabinol, thc}, month = {January}, number = {1}, pages = {335--346}, posted-at = {2008-05-26 18:26:31}, priority = {0}, title = {The inheritance of chemical phenotype in Cannabis sativa L.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12586720}, volume = {163}, year = {2003} }

TY - JOUR ID - citeulike:2835225 L3 - citeulike-article-id:2835225 TI - The inheritance of chemical phenotype in Cannabis sativa L. JF - Genetics VL - 163 IS - 1 SP - 335 EP - 346 AD - HortaPharm B.V., 1075 VS, Amsterdam, The Netherlands. SN - 0016-6731 N2 - Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F(1)'s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual F(1) plants were self-fertilized, and 10 inbred F(2) offspring were collected and analyzed. In all cases, a segregation of the three chemotypes (pure CBD, mixed CBD-THC, and pure THC) fitting a 1:2:1 proportion was observed. The CBD/THC ratio was found to be significantly progeny specific and transmitted from each F(1) to the F(2)'s derived from it. A model involving one locus, B, with two alleles, B(D) and B(T), is proposed, with the two alleles being codominant. The mixed chemotypes are interpreted as due to the genotype B(D)/B(T) at the B locus, while the pure-chemotype plants are due to homozygosity at the B locus (either B(D)/B(D) or B(T)/B(T)). It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC, respectively. The F(2) segregating groups were used in a bulk segregant analysis of the pooled DNAs for screening RAPD primers; three chemotype-associated markers are described, one of which has been transformed in a sequence-characterized amplified region (SCAR) marker and shows tight linkage to the chemotype and codominance. KW - cannabidiol KW - cannabinoid KW - cannabis KW - cdb KW - chemical KW - chemotype KW - codominance KW - cross KW - inheritance KW - locus KW - marker KW - phenotype KW - plant KW - rapd KW - sativa KW - scar KW - selfing KW - synthase KW - tetrahydrocannabinol KW - thc AU - de Meijer, EP AU - Bagatta, M AU - Carboni, A AU - Crucitti, P AU - Moliterni, VM AU - Ranalli, P AU - Mandolino, G PY - 2003/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/12586720 ER -