The human beta-globin replication initiation region consists of two modular independent replicators.

@article{citeulike:576081, abstract = {Previous studies have shown that mammalian cells contain replicator sequences, which can determine where DNA replication initiates. However, the specific sequences that confer replicator activity were not identified. Here we report a detailed analysis of replicator sequences that dictate initiation of DNA replication from the human beta-globin locus. This analysis suggests that the beta-globin replication initiation region contains two adjacent, redundant replicators. Each replicator was capable of initiating DNA replication independently at ectopic sites. Within each of these two replicators, we identified short, discrete, nonredundant sequences, which cooperatively determine replicator activity. Experiments with somatic cell hybrids further demonstrated that the requirements for initiation at ectopic sites were similar to the requirements for initiation within native human chromosomes. The replicator clustering and redundancy exemplified in the human beta-globin locus may account for the extreme difficulty in identifying replicator sequences in mammalian cells and suggest that mammalian replication initiation sites may be determined by cooperative sequence modules.}, address = {Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.}, author = {Wang, L. and Lin, C. M. and Brooks, S. and Cimbora, D. and Groudine, M. and Aladjem, M. I.}, citeulike-article-id = {576081}, issn = {0270-7306}, journal = {Mol Cell Biol}, keywords = {beta-globin, dna, initiation, origin, region, replication, replicator}, month = {April}, number = {8}, pages = {3373--3386}, posted-at = {2006-04-04 19:11:26}, priority = {3}, title = {The human beta-globin replication initiation region consists of two modular independent replicators.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15060158}, volume = {24}, year = {2004} }

TY - JOUR ID - citeulike:576081 L3 - citeulike-article-id:576081 N2 - Previous studies have shown that mammalian cells contain replicator sequences, which can determine where DNA replication initiates. However, the specific sequences that confer replicator activity were not identified. Here we report a detailed analysis of replicator sequences that dictate initiation of DNA replication from the human beta-globin locus. This analysis suggests that the beta-globin replication initiation region contains two adjacent, redundant replicators. Each replicator was capable of initiating DNA replication independently at ectopic sites. Within each of these two replicators, we identified short, discrete, nonredundant sequences, which cooperatively determine replicator activity. Experiments with somatic cell hybrids further demonstrated that the requirements for initiation at ectopic sites were similar to the requirements for initiation within native human chromosomes. The replicator clustering and redundancy exemplified in the human beta-globin locus may account for the extreme difficulty in identifying replicator sequences in mammalian cells and suggest that mammalian replication initiation sites may be determined by cooperative sequence modules. IS - 8 JF - Mol Cell Biol SN - 0270-7306 AD - Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA. EP - 3386 TI - The human beta-globin replication initiation region consists of two modular independent replicators. VL - 24 SP - 3373 KW - beta-globin KW - dna KW - initiation KW - origin KW - region KW - replication KW - replicator AU - Wang, L AU - Lin, CM AU - Brooks, S AU - Cimbora, D AU - Groudine, M AU - Aladjem, MI PY - 2004/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/15060158 ER -