Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface

by: GP Smith

Science, Vol. 228, No. 4705. (14 June 1985), pp. 1315-1317.

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Abstract

Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available. 10.1126/science.4001944

@article{citeulike:2614039, abstract = {Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available. 10.1126/science.4001944}, author = {Smith, G. P. }, citeulike-article-id = {2614039}, doi = {10.1126/science.4001944}, journal = {Science}, keywords = {historical, methods}, month = {June}, number = {4705}, pages = {1315--1317}, posted-at = {2008-03-31 04:32:55}, priority = {2}, title = {Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface}, url = {http://dx.doi.org/10.1126/science.4001944}, volume = {228}, year = {1985} }

RIS record

TY - JOUR ID - citeulike:2614039 L3 - citeulike-article-id:2614039 TI - Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface JF - Science VL - 228 IS - 4705 SP - 1315 EP - 1317 N2 - Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available. 10.1126/science.4001944 KW - historical KW - methods AU - Smith, GP PY - 1985/06/14/ UR - http://dx.doi.org/10.1126/science.4001944 ER -

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