Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma.

@article{citeulike:2620647, abstract = {Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients with IM, dysplasia, and cancer. These novel findings are compatible with the hypothesis that all stages of gastric carcinogenesis are fostered by persistent intracellular expression of H. pylori virulence genes, especially cagA inside MUC2-producing precancerous gastric cells and pleomorphic cancer cells.}, address = {Laboratory of Gastrointestinal and Liver Studies, Digestive Diseases Division, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.}, author = {Semino-Mora, C. and Doi, S. Q. and Marty, A. and Simko, V. and Carlstedt, I. and Dubois, A. }, citeulike-article-id = {2620647}, issn = {0022-1899}, journal = {J Infect Dis}, keywords = {pylori}, month = {April}, number = {8}, pages = {1165--1177}, posted-at = {2008-04-01 18:30:20}, priority = {2}, title = {Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12695995}, volume = {187}, year = {2003} }

TY - JOUR ID - citeulike:2620647 L3 - citeulike-article-id:2620647 TI - Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma. JF - J Infect Dis VL - 187 IS - 8 SP - 1165 EP - 1177 AD - Laboratory of Gastrointestinal and Liver Studies, Digestive Diseases Division, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA. SN - 0022-1899 N2 - Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients with IM, dysplasia, and cancer. These novel findings are compatible with the hypothesis that all stages of gastric carcinogenesis are fostered by persistent intracellular expression of H. pylori virulence genes, especially cagA inside MUC2-producing precancerous gastric cells and pleomorphic cancer cells. KW - pylori AU - Semino-Mora, C AU - Doi, SQ AU - Marty, A AU - Simko, V AU - Carlstedt, I AU - Dubois, A PY - 2003/04/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/12695995 ER -