Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.

by: RA Alm, LS Ling, DT Moir, BL King, ED Brown, PC Doig, DR Smith, B Noonan, BC Guild, BL deJonge, G Carmel, PJ Tummino, A Caruso, M Uria-Nickelsen, DM Mills, C Ives, R Gibson, D Merberg, SD Mills, Q Jiang, DE Taylor, GF Vovis, TJ Trust

Nature, Vol. 397, No. 6715. (14 January 1999), pp. 176-180.

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Abstract

Helicobacter pylori, one of the most common bacterial pathogens of humans, colonizes the gastric mucosa, where it appears to persist throughout the host's life unless the patient is treated. Colonization induces chronic gastric inflammation which can progress to a variety of diseases, ranging in severity from superficial gastritis and peptic ulcer to gastric cancer and mucosal-associated lymphoma. Strain-specific genetic diversity has been proposed to be involved in the organism's ability to cause different diseases or even be beneficial to the infected host and to participate in the lifelong chronicity of infection. Here we compare the complete genomic sequences of two unrelated H. pylori isolates. This is, to our knowledge, the first such genomic comparison. H. pylori was believed to exhibit a large degree of genomic and allelic diversity, but we find that the overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genomes) of the two strains are quite similar. Between 6 to 7% of the genes are specific to each strain, with almost half of these genes being clustered in a single hypervariable region.

@article{citeulike:407962, abstract = {Helicobacter pylori, one of the most common bacterial pathogens of humans, colonizes the gastric mucosa, where it appears to persist throughout the host's life unless the patient is treated. Colonization induces chronic gastric inflammation which can progress to a variety of diseases, ranging in severity from superficial gastritis and peptic ulcer to gastric cancer and mucosal-associated lymphoma. Strain-specific genetic diversity has been proposed to be involved in the organism's ability to cause different diseases or even be beneficial to the infected host and to participate in the lifelong chronicity of infection. Here we compare the complete genomic sequences of two unrelated H. pylori isolates. This is, to our knowledge, the first such genomic comparison. H. pylori was believed to exhibit a large degree of genomic and allelic diversity, but we find that the overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genomes) of the two strains are quite similar. Between 6 to 7\% of the genes are specific to each strain, with almost half of these genes being clustered in a single hypervariable region.}, address = {Astra Research Center Boston, Cambridge, Massachusetts 02139-4239, USA. richard.alm@arcb.us.astra.com}, author = {Alm, R. A. and Ling, L. S. and Moir, D. T. and King, B. L. and Brown, E. D. and Doig, P. C. and Smith, D. R. and Noonan, B. and Guild, B. C. and deJonge, B. L. and Carmel, G. and Tummino, P. J. and Caruso, A. and Uria-Nickelsen, M. and Mills, D. M. and Ives, C. and Gibson, R. and Merberg, D. and Mills, S. D. and Jiang, Q. and Taylor, D. E. and Vovis, G. F. and Trust, T. J. }, citeulike-article-id = {407962}, doi = {10.1038/16495}, issn = {0028-0836}, journal = {Nature}, keywords = {genomics, historical, pylori}, month = {January}, number = {6715}, pages = {176--180}, posted-at = {2008-04-03 09:07:04}, priority = {2}, title = {Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.}, url = {http://dx.doi.org/10.1038/16495}, volume = {397}, year = {1999} }

RIS record

TY - JOUR ID - citeulike:407962 L3 - citeulike-article-id:407962 TI - Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. JF - Nature VL - 397 IS - 6715 SP - 176 EP - 180 AD - Astra Research Center Boston, Cambridge, Massachusetts 02139-4239, USA. richard.alm@arcb.us.astra.com SN - 0028-0836 N2 - Helicobacter pylori, one of the most common bacterial pathogens of humans, colonizes the gastric mucosa, where it appears to persist throughout the host's life unless the patient is treated. Colonization induces chronic gastric inflammation which can progress to a variety of diseases, ranging in severity from superficial gastritis and peptic ulcer to gastric cancer and mucosal-associated lymphoma. Strain-specific genetic diversity has been proposed to be involved in the organism's ability to cause different diseases or even be beneficial to the infected host and to participate in the lifelong chronicity of infection. Here we compare the complete genomic sequences of two unrelated H. pylori isolates. This is, to our knowledge, the first such genomic comparison. H. pylori was believed to exhibit a large degree of genomic and allelic diversity, but we find that the overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genomes) of the two strains are quite similar. Between 6 to 7% of the genes are specific to each strain, with almost half of these genes being clustered in a single hypervariable region. KW - genomics KW - historical KW - pylori AU - Alm, RA AU - Ling, LS AU - Moir, DT AU - King, BL AU - Brown, ED AU - Doig, PC AU - Smith, DR AU - Noonan, B AU - Guild, BC AU - deJonge, BL AU - Carmel, G AU - Tummino, PJ AU - Caruso, A AU - Uria-Nickelsen, M AU - Mills, DM AU - Ives, C AU - Gibson, R AU - Merberg, D AU - Mills, SD AU - Jiang, Q AU - Taylor, DE AU - Vovis, GF AU - Trust, TJ PY - 1999/01/14/ UR - http://dx.doi.org/10.1038/16495 ER -

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